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Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
Published on: November 18, 2010
Quantification of Macrocirculation and Microcirculation in Brain Using Ultrasound Perfusion Imaging
Eline J Vinke1, Jens Eyding2, Chris de Korte3
1Department of Intensive Care, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
Objective:
The aim of this study was to investigate the feasibility of simultaneous visualization of the cerebral macrocirculation and microcirculation, using ultrasound perfusion imaging (UPI). In addition, we studied the sensitivity of this technique for detecting changes in cerebral blood flow (CBF).
Materials And Methods:
We performed an observational study in ten healthy volunteers. Ultrasound contrast was used for UPI measurements during normoventilation and hyperventilation. For the data analysis of the UPI measurements, an in-house algorithm was used to visualize the DICOM files, calculate parameter images and select regions of interest (ROIs). Next, time intensity curves (TIC) were extracted and perfusion parameters calculated.
Results:
Both volume- and velocity-related perfusion parameters were significantly different between the macrocirculation and the parenchymal areas. Hyperventilation-induced decreases in CBF were detectable by UPI in both the macrocirculation and microcirculation, most consistently by the volume-related parameters. The method was safe, with no adverse effects in our population.
Conclusions:
Bedside quantification of CBF seems feasible and the technique has a favourable safety profile. Adjustment of current method is required to improve its diagnostic accuracy. Validation studies using a 'gold standard' are needed to determine the added value of UPI in neurocritical care monitoring.
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