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Updated: Feb 13, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing
Armelle Marais1, Chantal Faure2, Bernard Bergey2
1UMR 1332, Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, Villenave d'Ornon, France. armelle.maraiks-colombel@inra.fr.
Abstract:
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing.
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