Related Experiment Video
Updated: Feb 13, 2026
In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
Published on: March 13, 2008
[Reproductive and developmental toxicity of 2- (2H-1, 2, 3-benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl)
Abstract:
Objective: To investigate the reproductive and developmental toxicity of 2- (2H-1, 2, 3-benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol in mice and to provide a basis for its risk assessment. Methods: The reproductive and developmental toxicity of 2- (2H-1, 2, 3-benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol was tested using the screening method of chemicals with reproductive and developmental toxicity in "Chemical Testing Method" (SEPA). After five days of adaptive feeding, 120 specific pathogen-free healthy Kunming mice (male/female ratio=1:1) were orally administered 0 (control) , 146, 292, and 584 mg/kg 2- (2H-1, 2, 3-benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol for two weeks. One male mouse was mated with one female mouse in a single cage. The day on which a vaginal plug was observed was defined as gestation day 0 (GD0). The exposure for female mice was sustained to four days postpartum and the exposure for male mice was sustained for two weeks after mating. The body weight, food intake, body length, tail length, and sex ratio were recorded and the reproductive index was calculated. The reproductive organs were weighed and subjected to histopathological examination. Results: The 584 mg/kg group had significantly lower body weight at weeks 5 and 6 and food intake at week 6 in male mice, uterus weight and uterus/body weight ratio in female mice, and body weight, body length, and tail length on day 0 in offspring compared with the control group (all P<0.05). The 292 mg/kg group had significantly lower testis weight of male mice and food intake of female mice at gestational week 2 than the control group (both P<0.05). The 146 mg/kg group had significantly lower food intake of female mice at gestational week 2 than the control group (P<0.05) . Conclusion: For male and female Kunming mice, the no observed adverse effect levels of 2- (2H-1, 2, 3, -benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol are both 146 mg/kg.
Insights
The reproductive toxicity of 2-(2H-1,2,3-benzotriazol-2-yl)-4-methyl-6-(2-methylpropen-2-yl)phenol was assessed in mice. The no observed adverse effect level for both male and female Kunming mice was determined to be 146 mg/kg.
Area of Science:
- Toxicology
- Reproductive Toxicology
- Developmental Toxicology
Background:
- 2-(2H-1,2,3-benzotriazol-2-yl)-4-methyl-6-(2-methylpropen-2-yl)phenol is a chemical requiring toxicological assessment.
- Understanding its reproductive and developmental effects is crucial for risk assessment.
Purpose of the Study:
- To investigate the reproductive and developmental toxicity of 2-(2H-1,2,3-benzotriazol-2-yl)-4-methyl-6-(2-methylpropen-2-yl)phenol in mice.
- To establish a basis for the risk assessment of this chemical.
Main Methods:
- Kunming mice were orally administered varying doses (0, 146, 292, 584 mg/kg) of the chemical.
- Reproductive parameters, organ weights, and histopathology were evaluated.
- Offspring development was monitored.
Main Results:
- Significant reductions in body weight, food intake, and organ weights were observed in higher dose groups (292 and 584 mg/kg).
- Offspring exposed to 584 mg/kg showed decreased body weight, length, and tail length.
- The no observed adverse effect level (NOAEL) was determined to be 146 mg/kg for both sexes.
Conclusions:
- The no observed adverse effect level (NOAEL) for 2-(2H-1,2,3-benzotriazol-2-yl)-4-methyl-6-(2-methylpropen-2-yl)phenol in male and female Kunming mice is 146 mg/kg.
- This study provides critical data for the risk assessment of this chemical.
![Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F60786.jpg&w=3840&q=50)
Related Concept Videos
01:13meta-Directing Deactivators: –NO2, –CN, –CHO, –CO2R, –COR, –CO2H
02:27Predicting Products: SN1 vs. SN2
With increased substitution on the alkyl halide,...
01:202° Amines to N-Nitrosamines: Reaction with NaNO2
01:20Criteria for Aromaticity and the Hückel 4n + 2 Rule
For the first time, Eric Hückel, a German chemical physicist, derived a set of structural features for a compound to be classified as aromatic. This is now known as Hückel’s rule or the 4n +...
02:14SN2 Reaction: Kinetics
In a chemical reaction, a relationship exists between the concentration of reactants and the rate at which the reaction proceeds. The study to measure this relationship is known as the kinetics of a chemical reaction. Kinetic studies are used to deduce the rate law of a chemical reaction, which provides information about the species involved during the transition state of the rate-determining step. Thus, kinetic studies help to derive the mechanism of a...
02:27SN2 Reaction: Mechanism
The presence of the more electronegative halogen in the substrate creates a polarized carbon-halide bond. The halide pulls the electron cloud generating an electrophilic center at the carbon atom. Thus, the carbon atom carries a partial positive charge while the halide has a...