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Updated: Feb 13, 2026

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
Published on: November 17, 2023
TopUp SERS Substrates with Integrated Internal Standard
Sophie Patze1, Uwe Huebner2, Karina Weber3,4
1Leibniz Institute of Photonic Technology e. V. (IPHT), Albert-Einstein-Str. 9, 07745 Jena, Germany. patzesophie@gmail.com.
Abstract:
Surface-enhanced Raman spectroscopy (SERS) is known as a molecular-specific and highly sensitive method. In order to enable the routine application of SERS, powerful SERS substrates are of great importance. Within this manuscript, a TopUp SERS substrate is introduced which is fabricated by a top-down process based on microstructuring as well as a bottom-up generation of silver nanostructures. The Raman signal of the support material acts as an internal standard in order to improve the quantification capabilities. The analyte molecule coverage of sulfamethoxazole on the surface of the nanostructures is characterized by the SERS signal evolution fitted by a Langmuir-Freundlich isotherm.
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