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Updated: Feb 13, 2026

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
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TopUp SERS Substrates with Integrated Internal Standard.

Sophie Patze1, Uwe Huebner2, Karina Weber3,4

  • 1Leibniz Institute of Photonic Technology e. V. (IPHT), Albert-Einstein-Str. 9, 07745 Jena, Germany. patzesophie@gmail.com.

Materials (Basel, Switzerland)
|March 3, 2018
PubMed
Summary
This summary is machine-generated.

A novel TopUp SERS substrate enhances quantification by using the support material's Raman signal as an internal standard. This development is crucial for routine applications of surface-enhanced Raman spectroscopy.

Keywords:
Langmuir–Freundlich isothermSERSTopUp SERS substratesulfamethoxazole

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Spectroscopy

Background:

  • Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity and molecular specificity.
  • Routine SERS applications require the development of powerful and reliable SERS substrates.
  • Accurate quantification in SERS is often limited by signal fluctuations.

Purpose of the Study:

  • To introduce a novel TopUp SERS substrate fabricated using a hybrid top-down and bottom-up approach.
  • To enhance the quantification capabilities of SERS by incorporating an internal standard.
  • To characterize analyte molecule coverage on the fabricated SERS substrate.

Main Methods:

  • Fabrication of the TopUp SERS substrate using microstructuring (top-down) and silver nanostructure generation (bottom-up).
  • Utilizing the Raman signal of the support material as an internal standard for improved quantification.
  • Characterizing sulfamethoxazole (analyte) coverage on nanostructures via SERS signal analysis.
  • Fitting SERS signal evolution to a Langmuir-Freundlich isotherm model.

Main Results:

  • Successful fabrication of the TopUp SERS substrate combining microstructuring and silver nanostructures.
  • Demonstration of the support material's Raman signal as an effective internal standard.
  • Quantitative analysis of sulfamethoxazole coverage, with signal evolution fitting the Langmuir-Freundlich isotherm.

Conclusions:

  • The TopUp SERS substrate offers enhanced quantification capabilities for SERS applications.
  • The integrated internal standard approach improves the reliability of SERS measurements.
  • This substrate design facilitates the routine and accurate application of SERS in chemical analysis.