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Updated: Feb 13, 2026

Proboscis Extension Response PER Assay in Drosophila
Published on: April 29, 2007
Serum starvation induces abnormal spindle location, RhoA delocalization, and extension of intercellular bridge with
Kazunori Nishimura1, Sumiko Watanabe2, Tsunehisa Kaku2
1a Department of Health Sciences, Graduate School of Medical Sciences , Kyushu University , Fukuoka City , Japan.
Abstract:
Serum starvation induces binucleation in HeLa cells, but the effects of serum starvation on mitosis and the significance of binucleation remain unknown. We investigated the effect of serum starvation on mitosis and analyzed the growth of binucleated cells. The frequency of binucleation caused by cytokinesis failure in DMEM without FBS (0% medium) was higher than that in DMEM with FBS (10% medium). In 0% medium, the metaphase spindle location was off-center, and RhoA localization significantly lacked symmetry. The frequency of the extension of intercellular bridge with the midbody in 0% medium was significantly higher than that in 10% medium. Moreover, all mononucleated mitotic cells caused bipolar mitosis and produced only mononucleated daughter cells, but binucleated cells produced various nucleated cells by multipolar mitosis in 0% medium. These results suggest that serum starvation may have various effects on mitosis, and binucleated cells may be related to formation of aneuploidy.
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