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Updated: Feb 13, 2026
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
Published on: December 18, 2017
Time course study of intestinal epithelial barrier disruption in acute mesenteric venous thrombosis
Shuofei Yang1, Jiaquan Chen1, Qihong Ni1
1Department of Vascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Background:
Acute superior mesenteric venous thrombosis (ASMVT) is an abdominal vascular condition. Early recanalization is essential to successful treatment. The aim of the study was to establish rabbit models of ASMVT and assess the time course of intestinal epithelial barrier disruption.
Methods:
After surgical exposure of superior mesenteric vein (Sham group), large-vessel (L-group) and small-vessel (S-group) models were established by endothelium damage, stenosis creation, and thrombin injection. At baseline, 6, 9, and 12 h, hemodynamic and serum parameters were tested. Serum from ASMVT patients diagnosed at 24, 36, 48, and 60 h from symptom onset was collected. Intestinal barrier disruption was assessed by tight junction (TJ) protein expression, morphology changes, and bacterial translocation. Mesenteric arteriospasm was measured by flow velocity and intestinal wet/dry weight ratio. The serum level of intestinal fatty acid-binding protein and endotoxin in patients was also measured as an indicator for intestinal barrier function.
Results:
Severe acidosis and lacticemia were observed in both the groups. The L-group experienced greater hemodynamic alteration than the S-group. Intestinal barrier disruption was detected by significantly decreased TJ protein expression, histology and ultrastructure injury of TJ, increased permeability, and bacterial translocation, at 9 h in the S-group and 12 h in the L-group. Secondary mesenteric arteriospasm occurred at the same time of complete intestinal barrier disruption and could be a significant cause of bowel necrosis. Significant increased level of intestinal fatty acid-binding protein and endotoxin was found in patients at 48 h in the S-group type and 60 h in the L-group type.
Conclusions:
The ASMVT animal models of both the types were first established. The loss of intestinal barrier function occurred at 6 h in the S-group model and 9 h in the L-group model. For clinical patients, the time window extended to 36 h in the S-group type and 48 h in the L-group type.
Insights
Acute superior mesenteric venous thrombosis (ASMVT) disrupts the intestinal barrier early. Rabbit models reveal barrier loss at 6-9 hours, with clinical implications for timely intervention in ASMVT patients.
Area of Science:
- Vascular Surgery
- Gastroenterology
- Pathophysiology
Background:
- Acute superior mesenteric venous thrombosis (ASMVT) is a critical abdominal vascular condition.
- Early recanalization is key for successful ASMVT treatment.
- Understanding intestinal barrier disruption is vital for managing ASMVT.
Purpose of the Study:
- Establish rabbit models of ASMVT to mimic human disease.
- Assess the time course of intestinal epithelial barrier disruption in ASMVT.
- Correlate animal model findings with human patient data.
Main Methods:
- Created large-vessel (L-group) and small-vessel (S-group) ASMVT rabbit models.
- Monitored hemodynamic and serum parameters at various time points.
- Evaluated intestinal barrier integrity via tight junction proteins, morphology, and bacterial translocation.
Main Results:
- Both ASMVT models showed severe acidosis and lacticemia.
- Intestinal barrier disruption (decreased TJ proteins, increased permeability, bacterial translocation) occurred by 9h (S-group) and 12h (L-group).
- Secondary mesenteric arteriospasm coincided with barrier disruption, suggesting a role in bowel necrosis.
Conclusions:
- Successfully established ASMVT rabbit models.
- Intestinal barrier function loss occurred early (6h in S-group, 9h in L-group) in animal models.
- Clinical ASMVT patients showed extended time windows for barrier dysfunction (36h S-group, 48h L-group).
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