Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

3.4K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
3.4K
Proton (¹H) NMR: Chemical Shift01:07

Proton (¹H) NMR: Chemical Shift

3.5K
Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei...
3.5K
NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

11.2K
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
11.2K
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

6.4K
Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
6.4K
NMR Spectroscopy of Benzene Derivatives01:34

NMR Spectroscopy of Benzene Derivatives

11.3K
Simple unsubstituted benzene has six aromatic protons, all chemically equivalent. Therefore, benzene exhibits only a singlet peak at δ 7.3 ppm in the 1H NMR spectrum. The observed shift is far downfield because the aromatic ring current strongly deshields the protons. Any substitution on the benzene ring makes the aromatic protons nonequivalent, and the protons split each other. The peak is, therefore, no longer a singlet and the splitting pattern and their associated coupling...
11.3K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

3.3K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robust single-scan ultraselective NMR.

Chemical communications (Cambridge, England)·2026
Same author

General Nuclear Magnetic Resonance Analysis Toolbox for Stats: A Comprehensive Module for Nuclear Magnetic Resonance-Based Chemometrics and Metabolomics.

Analytical chemistry·2026
Same author

Practical Guide and Best Practices for Diffusion NMR Processing With GNAT.

Magnetic resonance in chemistry : MRC·2026
Same author

Real-Time NMR Quantification of Paramagnetic Species during Chemical Reactions.

Analytical chemistry·2026
Same author

Full-Signal Ultrahigh-Resolution NMR by Parameter Estimation.

Analytical chemistry·2025
Same author

<sup>1</sup>H NMR Pure Shift Metabolomic Analysis of Black Tea.

Analytical chemistry·2025
Same journal

Application of Screen Printing in Perovskite Solar Devices.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

One-Shot Pd(II)-Catalyzed Multiple C-H Activation Enables Modular Construction of Fluorenylidene Oxindole-Based Multi(Polycyclic) Aromatic Enes.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Rapid Assembly of a Covalently Locked Organic Cage Revealing Symmetry-Matched Guest Recognition.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Expanding Cyanide-Bridged Weakly Coordinating Anions Through the Brominated Silver Salt Ag[BCNB<sup>Br</sup>].

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Photoexcited Nickel(0)-Catalyzed Direct Decarboxylative Cross-Coupling.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Divergent Total Syntheses of Bisnicalaterine Alkaloids Enabled by a Stereocontrolled Geissoschizol Synthesis.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: Feb 13, 2026

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

2.4K

PSYCHE Pure Shift NMR Spectroscopy.

Mohammadali Foroozandeh1, Gareth A Morris1, Mathias Nilsson1

  • 1School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|March 14, 2018
PubMed
Summary
This summary is machine-generated.

Pure Shift Yielded by Chirp Excitation (PSYCHE) enhances NMR spectral resolution by simplifying complex signals. This guide explains PSYCHE

Keywords:
NMR spectroscopyPSYCHEanalytical methodshomonuclear decouplingpure shift NMR

More Related Videos

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
07:54

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

Published on: December 15, 2011

13.5K
A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

15.2K

Related Experiment Videos

Last Updated: Feb 13, 2026

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

2.4K
Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
07:54

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

Published on: December 15, 2011

13.5K
A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

15.2K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Homonuclear coupling interactions in NMR spectra lead to signal splitting (multiplets), reducing spectral resolution.
  • Pure shift NMR techniques aim to suppress these homonuclear couplings, converting multiplets into singlets for clearer spectra.
  • Pure Shift Yielded by Chirp Excitation (PSYCHE) is a specific pure shift method known for its ease of use despite theoretical complexity.

Purpose of the Study:

  • To provide theoretical and practical insights into the PSYCHE NMR technique.
  • To elucidate the effects and importance of experimental parameters in PSYCHE.
  • To present recent improvements enhancing PSYCHE spectral purity and implementation frameworks.

Main Methods:

  • Detailed explanation of the PSYCHE technique, involving selective inversion of passive spins to negate homonuclear coupling effects.
  • Analysis of key experimental parameters influencing PSYCHE performance and spectral quality.
  • Demonstration of PSYCHE implementation in 1D and 2D NMR spectroscopy experiments.

Main Results:

  • Insights into the theoretical underpinnings and practical application of PSYCHE.
  • Identification of critical experimental parameters for optimizing PSYCHE performance.
  • Presentation of improved PSYCHE methods yielding enhanced spectral purity.

Conclusions:

  • PSYCHE is a powerful yet accessible pure shift NMR technique for improving spectral resolution.
  • Understanding experimental parameters is crucial for effective PSYCHE implementation.
  • Recent advancements have further enhanced the utility and spectral quality of PSYCHE.