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Related Concept Videos

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Porin Insertion in the Outer Mitochondrial Membrane01:12

Porin Insertion in the Outer Mitochondrial Membrane

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Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
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Classification of Titrimetric Analysis Based on Reaction Types01:01

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Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
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Insertion of Single-pass Transmembrane Proteins in the RER01:26

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Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
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Insertion of Multi-pass Transmembrane Proteins in the RER01:29

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The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
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A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation
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Insert-based microfluidics for 3D cell culture with analysis.

Chengpeng Chen1, Alexandra D Townsend1, Elizabeth A Hayter1

  • 1Department of Chemistry, Saint Louis University, 3501 Laclede Ave., St. Louis, MO, 63103, USA.

Analytical and Bioanalytical Chemistry
|March 15, 2018
PubMed
Summary
This summary is machine-generated.

We developed a novel insert-based microfluidic device for scalable 3D cell culture. This system allows customized cell seeding and easy integration with detection modules for in vitro studies.

Keywords:
3D printingBioanalytical methodsCell systemsMicrofluidicsSingle-cell analysis

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Biology

Background:

  • 3D cell culture models are crucial for mimicking in vivo environments.
  • Existing microfluidic devices often lack scalability and modularity for diverse cell studies.

Purpose of the Study:

  • To present an insert-based microfluidic device for scalable and multiplexable 3D cell culture.
  • To enable seamless integration with downstream detection modules for flow-based investigations.

Main Methods:

  • Fabrication of laser-cut inserts with electrospun fibers for 3D cell scaffolding.
  • Assembly of inserts into a 3D-printed microfluidic device for flow studies.
  • Culturing endothelial cells and macrophages, and stimulating macrophages under flow conditions.

Main Results:

  • Demonstrated successful cell adhesion and culture on fibrous inserts under flow.
  • Observed significant effects of fibrous scaffolds and flow on macrophage TNF-α production kinetics.
  • Integrated the cell culture module with a downstream absorbance detection scheme.

Conclusions:

  • The insert-based microfluidic device offers a versatile platform for in vivo-like 3D cell culture.
  • This approach facilitates customized cell seeding, scalability, and multiplexing.
  • Enables advanced in vitro studies with integrated online detection capabilities.