Related Experiment Video
Updated: Feb 13, 2026

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
Published on: February 2, 2018
Determination of Food Oxalates Using Silica-Titania Xerogel Modified with Eriochrome Cyanine R
Maria A Morosanova1, Zahar V Samodelov2, Elena I Morosanova3
1Analytical Chemistry Division, Chemistry Department, Lomonosov Moscow State University, Moscow 119234, Russia. m.a.morosanova@gmail.com.
Abstract:
The interaction of silica-titania xerogel with triphenylmethane dyes (pyrocatechol violet, chrome azurol S, eriochrome cyanine R) has been investigated to create a new sensor material for solid phase spectrophotometric determination of food oxalates. The complex forming reaction between xerogel incorporated titanium(IV) and triphenylmethane dyes has been studied; half-reaction periods, complex composition, equilibrium constants, and xerogel sorption capacity have been calculated for each dye. Eriochrome cyanine R (ECR) is characterized by the shortest half-reaction period, the smallest equilibrium constant, and the greatest capacity; it has been chosen for the sensor material construction because titanium(IV)-ECR complex is formed faster and can be destroyed easier than other studied complexes. The interaction of this sensor material with oxalates has been described: the presence of oxalates causes sensor material discoloration and the absorbance is used as analytical signal. The analytical range is 35-900 mg/L (LOD 10.5 mg/L, n = 7). High concentrations of interfering inorganic anions, organic acids, and sucrose did not affect oxalate determination. Proposed solid phase spectrophotometric procedure has been successfully applied for the determination of oxalates in food samples (sorrel, spinach, parsley, ginger, and black pepper) and the results are in good agreement with HPLC oxalate determination.
More Related Videos
Related Concept Videos
Regulation of Food Intake
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Microorganisms in Agriculture and Food industry
Modified Boxplots
However, the box plot does not tell the reader about outliers - values that lie far from the center of the data. We can modify the standard box and whisker plot to identify the outliers and visualize the actual spread of the data in a sample.
Initially, we calculate the adjusted...
Determining Order of Reaction
Determination

