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Updated: Feb 13, 2026
06:34
Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
Published on: March 8, 2024
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Translating Developmental Principles to Generate Human Gastric Organoids
Alexandra K Eicher1, H Matthew Berns1, James M Wells1,2,3
1Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
Cellular and Molecular Gastroenterology and Hepatology
|March 20, 2018
Summary
Human gastric organoids (HGOs) were developed from pluripotent stem cells to model stomach development and disease. This 3D model system offers new avenues for studying gastric biology and potential therapeutic applications.
Area of Science:
- Gastroenterology and Developmental Biology
- Stem Cell Biology and Regenerative Medicine
- Human Organoid Technology
Background:
- Gastric diseases like peptic ulcers and cancer are common, yet stomach cellular biology is poorly understood.
- Existing animal models have limitations due to significant structural and physiological differences from human stomachs.
- Understanding human gastric development and lineage differentiation is crucial for addressing these diseases.
Purpose of the Study:
- To establish a human-specific model system for studying gastric development, function, and disease.
- To detail the generation of human gastric organoids (HGOs) from human pluripotent stem cells.
- To highlight the potential applications of HGOs in research and clinical settings.
Main Methods:
- Directed differentiation of human pluripotent stem cells in a 3D environment.
- Step-wise differentiation protocol mimicking in vivo temporal-spatial signaling dynamics of stomach development.
- Generation of HGOs recapitulating key aspects of human gastric tissue.
Main Results:
- Successful generation of human gastric organoids (HGOs) in vitro.
- HGOs serve as a viable model for studying human gastric development and disease.
- HGOs facilitate the study of epithelial and nonepithelial cell interactions, including endothelial, neuronal, and mesenchymal cells.
Conclusions:
- Human gastric organoids (HGOs) provide an essential in vitro model for understanding human stomach biology.
- HGOs offer significant potential for genetic modeling, drug screening, and future therapeutic applications.
- Further research into epithelial-mesenchymal interactions within HGOs is vital for understanding stomach development and function.
Keywords:
3-D, 3-dimensionalBMP, bone morphogenetic proteinDirected DifferentiationECL, enterochromaffin-likeENCC, enteric neural crest cellENS, enteric nervous systemEndodermGI, gastrointestinalGastric DevelopmentHDGC, hereditary diffuse gastric cancerHGO, human gastric organoidOrganoidsPSC, pluripotent stem cellPluripotent Stem CellsShh, Sonic hedgehoge, embryonic dayhPSC, human pluripotent stem cell
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