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Related Experiment Video

Updated: Feb 13, 2026

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Published on: June 19, 2019

Aptamer-facilitated mass cytometry.

Gleb G Mironov¹, Alexandre Bouzekri², Jessica Watson²

¹Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie-Curie, Ottawa, K1N 6N5, Canada.

Analytical and Bioanalytical Chemistry

|March 21, 2018

Summary

This summary is machine-generated.

Biotinylated aptamers can be used with mass cytometry for cell analysis, matching antibody performance. This technique offers high channel counts and low noise for precise cell characterization.

Keywords:

Aptamers Flow cytometry Mass cytometry PTK7

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

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Published on: October 6, 2022

Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Mass cytometry is an advanced cell analysis method using elemental tags instead of fluorophores.
It offers advantages like high channel capacity (up to 100) and reduced background noise.
Traditional methods often require compensation, which mass cytometry negates.

Purpose of the Study:

To evaluate the efficacy of biotinylated aptamers for cell characterization using mass cytometry.
To compare aptamer-based detection with conventional antibody-based methods.
To demonstrate aptamers as a viable alternative for mass cytometry applications.

Main Methods:

Utilized a biotinylated aptamer targeting the human PTK7 receptor.
Employed metal-labeled neutravidin for aptamer detection.
Analyzed human acute lymphoblastic leukemia (PTK7 positive) and human Burkitt's lymphoma (PTK7 negative) cells.
Mass cytometry involved cell ionization in inductively coupled plasma (ICP) and time-of-flight mass spectrometry (MS) analysis.

Main Results:

Biotinylated aptamers successfully identified PTK7 receptor expression on target cells.
The aptamer-based method demonstrated comparable performance to commercially available antibodies.
Proof-of-principle experiments confirmed the utility of aptamers in mass cytometry.

Conclusions:

Biotinylated aptamers are effective tools for mass cytometry analysis.
This approach provides a robust alternative to antibodies for cell biomarker detection.
The findings support the broader application of aptamers in high-dimensional single-cell analysis.