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Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
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Identification of Chromothripsis in Biopsy Using SNP-Based Microarray
Veronica Ortega1, Christina Mendiola1, Gopalrao V N Velagaleti2
1Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 23, 2018
Summary
Cancer cells can evolve through catastrophic genomic events like chromothripsis, not just gradual changes. SNP microarrays offer an alternative to sequencing for detecting these complex rearrangements in tumor samples.
Area of Science:
- Genomics
- Cancer Biology
- Evolutionary Medicine
Background:
- Genomic instability is a hallmark of cancer.
- Cancer evolution is typically viewed as a gradual process.
- Recent research highlights catastrophic genomic events like chromothripsis.
Purpose of the Study:
- To explore chromothripsis as an alternative cancer evolution mechanism.
- To compare detection methods for chromothripsis.
Main Methods:
- Review of recent studies on chromothripsis and cancer evolution.
- Discussion of sequencing and single-nucleotide polymorphism (SNP)-based microarray methods for detection.
Main Results:
- Chromothripsis can cause complex genomic rearrangements in a single event.
- Sequencing is ideal for chromothripsis detection.
- SNP-based microarrays are a viable alternative, especially for challenging biopsy samples.
Conclusions:
- Chromothripsis represents a distinct evolutionary pathway in cancer.
- SNP microarrays provide a practical method for detecting chromothripsis when sequencing is limited.
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