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Ten steps to get started in Genome Assembly and Annotation.
Victoria Dominguez Del Angel1, Erik Hjerde2, Lieven Sterck3,4
1Institut Français de Bioinformatique, UMS3601-CNRS, Université Paris-Saclay, Orsay, 91403, France.
F1000Research
|March 24, 2018
Summary
This guide provides 10 essential steps for researchers to begin genome assembly and annotation projects. It covers everything from DNA quality and sequencing to data management and FAIR principles for genome data.
Area of Science:
- Genomics
- Bioinformatics
- Molecular Biology
Background:
- Genome assembly and annotation are fundamental processes in modern biological research.
- Researchers require clear, stable, and comprehensive guidelines to initiate these complex projects.
- Capacity building initiatives are crucial for advancing genomic research capabilities.
Purpose of the Study:
- To present a 10-step framework for researchers new to genome assembly and annotation.
- To provide broadly applicable and stable guidelines for general genome project workflows.
- To emphasize critical aspects including DNA quality, sequencing technologies, and data management.
Main Methods:
- Discussion of intrinsic genome properties and the importance of high-quality DNA.
- Detailed explanation of various sequencing technologies and general genome assembly workflows.
- Coverage of structural and functional annotation, including transposable elements.
Main Results:
- A comprehensive 10-step guide for initiating and completing genome assembly and annotation projects.
- Inclusion of often-omitted aspects like transposable element annotation.
- Emphasis on data management and adherence to Findable, Accessible, Interoperable, and Reusable (FAIR) principles.
Conclusions:
- The provided guidelines facilitate researchers' entry into genome assembly and annotation.
- Adherence to these steps ensures robust, reproducible, and FAIR-compliant genomic data.
- This resource supports capacity building in genomics research.
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