Related Experiment Video
Updated: Feb 12, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
Identification of small ORF-encoded peptides in mouse serum
Yaqin Deng1,2, Adekunle Toyin Bamigbade1, Mirza Ahmed Hammad1
11National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
Abstract:
Identification of the coding elements in the genome is fundamental to interpret the development of living systems and species diversity. Small peptides (length < 100 amino acids) have played an important role in regulating the biological metabolism, but their identification has been limited by their size and abundance. Serum is the most important body fluid and is full of small peptides. In this study, we have established a small ORF-encoded peptides (SEPs) database from mouse GENCODE release. This database provides about half a million putative translated SEPs in mouse. We also extract serum proteins from wild type and ob/ob mice, and collect the low molecular weight proteins for mass spectrometric analysis. More than 50 novel SEPs have been discovered. Several SEPs are further verified by biochemical method with newly raised antibodies. These novel SEPs enhance the knowledge about the complexity of serum and provide new clues for the annotation and functional analysis of genes, especially the noncoding elements in the genome.
More Related Videos
Related Concept Videos
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Peptide Bonds
Encoding
Automatic processing involves the encoding of details like time, space, frequency, and the meaning of words, usually done without conscious...
Serum Studies: Renal Function Tests
Serum Laboratory Studies, Stool Test, Breath Test
Methods of Classification and Identification

