Development of Certified Matrix-Based Reference Material as a Calibrator for Genetically Modified Rice G6H1 Analysis

Area of Science:

• Agricultural Biotechnology
• Food Science
• Analytical Chemistry

Background:

• Accurate quantification of genetically modified organisms (GMOs) is crucial for regulatory compliance and consumer safety.
• Certified Reference Materials (CRMs) are indispensable tools for validating GMO detection methods and ensuring accurate quantification.

Purpose of the Study:

• To develop and characterize matrix-based CRMs for transgenic rice event G6H1.
• To provide reliable standards for the accurate monitoring and labeling of G6H1 GMO rice.

Main Methods:

• Production of G6H1 CRMs by mixing homozygous G6H1 and non-GM rice powders at specific mass ratios.
• Evaluation of between-bottle and within-bottle homogeneity.
• Assessment of DNA stability over time.
• Characterization of CRMs using mass fraction and droplet digital PCR (ddPCR) for copy number ratio determination.

Main Results:

• Three G6H1 CRMs (G6H1a, G6H1b, G6H1c) were successfully produced with precise mass fraction ratios.
• Property values were determined as (49.825 ± 0.448) g/kg, (9.967 ± 1.757) g/kg, and (4.986 ± 1.274 g/kg.
• ddPCR analysis yielded copy number ratios of (5.01 ± 0.08)%, (1.06 ± 0.22)%, and (0.53 ± 0.11)%.

Conclusions:

• The developed G6H1 matrix-based CRMs are of high quality with accurate characterization values.
• These CRMs are suitable for use as calibrators in GMO rice inspection and monitoring.
• The CRMs will aid in evaluating new analytical methods and equipment for G6H1 event detection.