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Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
Published on: September 15, 2014
MicroRNA profiling during directed differentiation of cortical interneurons from human-induced pluripotent stem cells
Jiajie Tu1,2, Dandan Cao1, Lu Li2
1Ministry of Education Key Laboratory for Regenerative Medicine (CUHK-Jinan University) School of Biomedical Sciences Faculty of Medicine The Chinese University of Hong Kong Hong Kong SAR China.
Abstract:
Induced pluripotent stem cells (iPSCs) are useful for modeling neuron development and related diseases. Cortical interneurons are essential players in neuropsychiatric diseases such as autism. miRNAs are a class of pivotal regulators in neural differentiation. Using a previously established model of cortical interneuron differentiation from human embryonic stem cells, we profiled miRNAs involved in differentiation from human iPSCs. A number of miRNAs were modulated in the differentiation process. This study captured the temporal in vitro neurogenesis from iPSCs to mature cortical interneurons. The specific miRNAs identified at each stage of differentiation are of potential use for drug discovery and prospective clinical applications.
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