Optimization of PLGA formulation containing protein or peptide-based antigen: Recent advances

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Immunology

Background:

• Protein and peptide antigens are crucial for developing custom vaccines and therapeutic immune responses.
• Biodegradable poly(lactic-co-glycolic acid) (PLGA) is widely explored for delivering protein and peptide-based therapeutics.
• Challenges remain in maintaining antigen integrity during PLGA formulation, release, and storage.

Purpose of the Study:

• To review the properties and preparation techniques of PLGA particles for antigen delivery.
• To summarize strategies for enhancing protein and peptide antigen stability within PLGA systems.
• To discuss approaches for stabilizing antigens during encapsulation, release, and storage.

Main Methods:

• Literature review of poly(lactic-co-glycolic acid) (PLGA) particle preparation and antigen stabilization techniques.
• Analysis of factors affecting protein/peptide antigen integrity during formulation and storage.
• Summary of various stabilization strategies for encapsulated antigens.

Main Results:

• PLGA particles offer a versatile platform for protein and peptide antigen delivery.
• Antigen instability during encapsulation, release, and storage is a significant challenge.
• Optimized formulation and stabilization strategies are essential for preserving antigen structure and function.

Conclusions:

• Effective stabilization of protein antigens within PLGA particles is critical for successful clinical translation.
• Further research into advanced formulation and stabilization techniques is needed to maximize the therapeutic potential of PLGA-based antigen delivery systems.