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Updated: Feb 12, 2026

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Published on: January 17, 2014
A highly efficient dual-diazonium reagent for protein crosslinking and construction of a virus-based gel
Dejun Ma1, Jie Zhang, Changyu Zhang
1State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China.
Abstract:
A new bench-stable reagent with double diazonium sites was designed and synthesized for protein crosslinking. Based on the highly efficient diazonium-Tyr coupling reaction, a direct mixture of the reagent and tobacco mosaic virus led to the formation of a new hydrogel, which could be degraded by chemicals and could be used to encapsulate small molecules for sustained release. Because plant viruses exhibit many chemical characteristics like protein labelling and nucleic acid packaging, the virus-based hydrogel will have large chemical space for further functionalization. Besides, this dual-diazonium reagent should be a generally useful crosslinker for chemical biology and biomaterials.
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