OPTIMIZATION AND VALIDATION OF HPLC METHOD FOR TETRAMETHRIN DETERMINATION IN HUMAN SHAMPOO FORMULATION

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Tetramethrin is an antiparasitic agent used in human shampoos.
• Accurate quantification of tetramethrin is essential for quality control and safety.

Purpose of the Study:

• To optimize and validate a high-performance liquid chromatography (HPLC) method for tetramethrin determination.
• To establish a reliable analytical technique for tetramethrin in pharmaceutical formulations.

Main Methods:

• Method development involved optimizing column type, oven temperature, and mobile phase composition.
• Validation included assessing linearity, accuracy (mean recovery), and specificity.
• Diode array detection (DAD) was employed for tetramethrin detection.

Main Results:

• Optimal separation was achieved using a Supelcosil TM LC-18- DB column with a methanol:water mobile phase (78:22, v/v) at 30°C and 0.8 mL/min flow rate.
• The method demonstrated excellent linearity (r² = 0.9997) and high accuracy (100.09% mean recovery).
• Detection was set at 220 nm.

Conclusions:

• The developed HPLC-DAD method is suitable for the routine analysis of tetramethrin in antiparasitic human shampoos.
• This validated method provides a robust tool for pharmaceutical research and quality control involving tetramethrin.