IN VITRO ANTIFUNGAL ACTIVITY OF 2,4-DIHYDROXY-N-(3-THIOXO-3H- 1,2,4-DITHIAZOL-5-YL)BENZENECARBOTHIOAMIDE

An increase in infections due to non albicans species of Candida has been observed in the recent years. The aims of this study were to determine the antifungal activity of 2,4-dihydroxy-N-(3-thioxo-3H-l1,2,4-dithiazol-5-yl)benzenecarbothioamide (DNTDB) against C. albicans, non-C. albicans, dermatophytes, and molds and to evaluate the enzymatic activity of C. albicans strains. We used reference strans C. albicans 10231 ATCC, 200 C. albicans strains, 100 non-C. albicans, 19 dermatophyte strains, and 21 mold strains isolated from different ontocenoses from patients. DNTDB revealed a mean minimum inhibitory concentration (MIC) of 12.5 pg/mL against the reference C. albicans 10231 ATCC strain on Sabouraud agar (SA) and of 6.5 pg/mL on Roswell Park Memorial Institute (RPMI) medium. The mean MIC for C. albicans isolates was of 22.01 ± 7.5 μg/mL on SA, 17.8 ± 7.4 μg/mL on yeast nitrogen base (YNB), and 16.9 ± 7.9 μg/mL on RPMI medium. The mean MIC for non-C. albicans isolates was of 22.4 ± 12.4 μg/mL on SA, 18.2 ± 8.6 μg/mL on YNB and 15.2 ± 9.03 sg/mL on RPMI. Against Trichophyton mentagrophytes v. granulosum, the mean MIC was 10.9 ± 2.04 μg/mL after 5 days of incubation and 21.9 ± 3.8 μg/mL after 15 days, while Trichophyton mentagrophytes . inteiligitale showed a mean MIC of 13.3 ± 5.5 μg/mL and of 20.3 ± 6.1. μg/mL after the same incubation periods, respectively. DNTDB manifested a MIC over the test range of 25-100 μg/mL for molds after 5 days of incubation and inhibited the enzymatic activity of Candida strains. It seems, the new DNTDB demonstrates potential antifungal activity against yeast-like fungus strains, dermatophytes, and molds in vitro.