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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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The technique...
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Model-Independent Approaches for Pharmacokinetic Data: Noncompartmental Analysis00:59

Model-Independent Approaches for Pharmacokinetic Data: Noncompartmental Analysis

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Noncompartmental analyses offer an alternative method for describing drug pharmacokinetics without relying on a specific compartmental model. In this approach, the drug's pharmacokinetics are assumed to be linear, with the terminal phase log-linear. This assumption allows for simplified analysis and interpretation of the drug's behavior in the body.
One important characteristic of noncompartmental analyses is that drug exposure increases proportionally with increasing doses. This...
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Analysis Methods of Pharmacokinetic Data: Model and Model-Independent Approaches01:14

Analysis Methods of Pharmacokinetic Data: Model and Model-Independent Approaches

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Drug disposition in the body is a complex process and can be studied using two major approaches: the model and the model-independent approaches.
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What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
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Frustration and Conflict: Approach-Approach, Approach-Avoidance01:20

Frustration and Conflict: Approach-Approach, Approach-Avoidance

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Frustration occurs when people are obstructed or prevented from achieving a desired goal or fulfilling a perceived need. For example, when someone's input is ignored in a discussion, it can lead to feelings of frustration. Conflict, however, arises from opposing interests, goals, or actions. Conflicts can take various forms based on the nature of these opposing desires or goals.
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Related Experiment Video

Updated: Feb 12, 2026

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
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Proteomic approaches beyond expression profiling and PTM analysis.

Jiaqi Fu1, Mei Wu1, Xiaoyun Liu2

  • 1Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Rd., Haidian District, Beijing, 100871, China.

Analytical and Bioanalytical Chemistry
|April 12, 2018
PubMed
Summary
This summary is machine-generated.

This review highlights novel proteomic methods beyond protein expression and modifications. These techniques offer new ways to study protein functions, including proximity labeling and activity-based profiling.

Keywords:
Activity-based protein profilingNewly synthesized proteinsProximity-based labelingTerminal proteomics

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Area of Science:

  • Proteomics
  • Molecular Biology
  • Biochemistry

Background:

  • Cellular functions rely on proteins, whose properties like expression and post-translational modifications (PTMs) are dynamically regulated.
  • Traditional proteomic studies primarily focus on quantifying protein abundance and PTMs.

Purpose of the Study:

  • To review recent advancements in proteomic approaches that explore protein functions beyond expression levels and PTMs.
  • To highlight innovative techniques for understanding protein roles in cellular processes.

Main Methods:

  • Proximity-based labeling techniques.
  • Analysis of protein termini and newly synthesized proteins.
  • Activity-based protein profiling (ABPP).

Main Results:

  • These advanced methods provide new insights into protein functions.
  • Proximity labeling maps protein interactions in situ.
  • ABPP reveals active enzymes within complex biological systems.

Conclusions:

  • Emerging proteomic strategies offer a functional perspective on proteins.
  • These techniques complement traditional methods, expanding our understanding of cellular mechanisms.