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Updated: Feb 11, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
[Carriage of Staphylococcus aureus among food service workers]
María Paula Alarcón-Lavín1, Carolina Oyarzo2, Carlos Escudero2
1Departamento de Nutrición y Salud Pública, Facultad de Ciencias de la Salud y los Alimentos, Universidad del Bío-Bío, Chillán, Chile.
Abstract:
Background Staphylococcus aureus produces 11 serotypes of endotoxins that may cause food poisoning. Aim To determine the prevalence of type A enterotoxigenic Staphylococcus aureus carriage among food service workers in Chillan, Chile. Material and Methods Pharyngeal swabs were obtained from 100 food service workers and were cultured in Agar plates. After identifying the presence of Staphylococcus aureus, DNA was extracted to identify type A toxin by conventional PCR. Results Thirty eight percent of samples were colonized with Staphylococcus aureus. Among these, 26% were toxin A producers. Conclusions Half of the sampled workers carried Staphylococcus aureus and a quarter of these produced type A enterotoxin.
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