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Updated: Feb 11, 2026
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Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
Published on: April 11, 2013
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Arcopilus aureus, a Resveratrol-Producing Endophyte from Vitis vinifera
Vagish Dwibedi1, Sanjai Saxena2
1Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India.
Applied Biochemistry and Biotechnology
|April 15, 2018
Summary
Researchers isolated resveratrol-producing endophytic fungi from Vitis vinifera grapes. The fungus Arcopilus aureus was identified as a top producer, offering potential for natural resveratrol sourcing.
Area of Science:
- Microbiology
- Phytochemistry
- Pharmacognosy
Background:
- Resveratrol, known for its health benefits, is a valuable compound in drug development.
- Endophytic fungi residing within plants can produce bioactive metabolites.
- Vitis vinifera (grapevine) is a rich source of potential endophytic microorganisms.
Purpose of the Study:
- To isolate and identify endophytic fungi from various Vitis vinifera cultivars capable of producing resveratrol.
- To screen these isolates for their resveratrol yield.
- To characterize the most promising resveratrol-producing fungal strain.
Main Methods:
- Isolation of endophytic fungi from Vitis vinifera stems and leaves.
- Phytochemical screening for phenolics.
- Resveratrol extraction using ethyl acetate.
- Confirmation via Thin Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC).
- Morphological and molecular identification of fungal isolates.
Main Results:
- 53 endophytic fungal isolates were obtained from five Vitis vinifera varieties.
- 29 isolates showed positive results for phenolics.
- Resveratrol was confirmed by TLC (Rf 0.69) and HPLC.
- The isolate #12VVLPM yielded the highest resveratrol concentration (89.1 μg/ml).
- #12VVLPM was identified as Arcopilus aureus, belonging to the seven genera identified.
Conclusions:
- Endophytic fungi from Vitis vinifera are a viable source for resveratrol production.
- Arcopilus aureus (#12VVLPM) is a significant producer of resveratrol.
- This discovery opens avenues for sustainable and natural resveratrol sourcing through fungal biotechnology.
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