Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multi-input and Multi-variable systems01:22

Multi-input and Multi-variable systems

428
Cruise control systems in cars are designed as multi-input systems to maintain a driver's desired speed while compensating for external disturbances such as changes in terrain. The block diagram for a cruise control system typically includes two main inputs: the desired speed set by the driver and any external disturbances, such as the incline of the road. By adjusting the engine throttle, the system maintains the vehicle's speed as close to the desired value as possible.
In the absence of...
428
Multi-Step Reactions02:31

Multi-Step Reactions

8.8K
Chemical reactions often occur in a stepwise fashion involving two or more distinct reactions taking place in a sequence. A balanced equation indicates the reacting species and the product species, but it reveals no details about how the reaction occurs at the molecular level. The reaction mechanism (or reaction path) provides details regarding the precise, step-by-step process by which a reaction occurs. Each of the steps in a reaction mechanism is called an elementary reaction. These...
8.8K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.9K
Applications of Logarithms01:28

Applications of Logarithms

288
Logarithmic functions are powerful tools for simplifying the mathematical representation of phenomena involving exponential changes. Their ability to convert multiplicative relationships into additive ones is especially valuable in various scientific and engineering contexts. One notable application of logarithms is measuring sound intensity, specifically through the decibel (dB) scale used in acoustics.Sound intensity levels vary over an extensive range, from the faintest audible whisper to...
288
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.1K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.1K
Radiation: Applications01:17

Radiation: Applications

1.8K
The average temperature of Earth is the subject of much current discussion. Earth is in radiative contact with both the Sun and dark space; it receives almost all its energy from the radiation of the Sun and reflects some of it into outer space. Dark space is very cold, about 3 K, so Earth radiates energy into it. For instance, heat transfer occurs from soil and grasses, the rate of which can be so rapid that frost can occur on clear summer evenings, even in warm latitudes.
The average...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring Cerumen as an Alternative Matrix for Drug Detection and Quantification: Method Development and Application in Clinical Samples.

Drug testing and analysis·2026
Same author

Untargeted metabolomics reveals key biochemical shifts during industrial Muscat of Alexandria alcoholic fermentation.

Food chemistry·2026
Same author

Toward minimally invasive metabolomics: GC-MS metabolic fingerprints of dried blood microsamples in comparison to plasma.

The Analyst·2025
Same author

Oxygen attachment dissociation (OAD) MS/MS in the identification of positional isomers of dysregulated lipids detected in an ethanol exposure metabolomics study in mice.

Metabolomics : Official journal of the Metabolomic Society·2025
Same author

Metabolic Profiling: A Perspective on the Current Status, Challenges, and Future Directions.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Quality Control and Validation Issues in LC-MS-Based Metabolomics.

Methods in molecular biology (Clifton, N.J.)·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 11, 2026

Author Spotlight: Advancing Metabolomics Analysis of Rare Hematopoietic Stem Cells
08:28

Author Spotlight: Advancing Metabolomics Analysis of Rare Hematopoietic Stem Cells

Published on: February 23, 2024

2.6K

HILIC-MS/MS Multi-Targeted Method for Metabolomics Applications.

Christina Virgiliou1, Helen G Gika2, Georgios A Theodoridis3

  • 1Laboratory of Forensic Medicine and Toxicology, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece. cr_virgi@hotmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|April 15, 2018
PubMed
Summary
This summary is machine-generated.

This study presents a new method for absolute quantification of about 100 polar metabolites using hydrophilic interaction liquid chromatography (HILIC) and mass spectrometry. This technique aids in understanding biochemical changes in various conditions.

Keywords:
HILIC-MS/MSMetabolic profilingPolar analytesTargeted metabolomics

More Related Videos

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.4K
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.7K

Related Experiment Videos

Last Updated: Feb 11, 2026

Author Spotlight: Advancing Metabolomics Analysis of Rare Hematopoietic Stem Cells
08:28

Author Spotlight: Advancing Metabolomics Analysis of Rare Hematopoietic Stem Cells

Published on: February 23, 2024

2.6K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.4K
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.7K

Area of Science:

  • Metabolomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Metabolomics identifies and quantifies metabolites to study biochemical activity changes.
  • Targeted profiling assays, especially LC-MS/MS, are increasingly popular for metabolite quantification.

Purpose of the Study:

  • To describe a method for absolute quantification of approximately 100 key polar metabolites.
  • To establish a robust method for analyzing diverse metabolite classes.

Main Methods:

  • Utilized hydrophilic interaction liquid chromatography (HILIC) coupled with ultra-high performance liquid chromatography (UHPLC).
  • Employed a triple-quadrupole mass spectrometer with positive and negative electrospray ionization modes.
  • Focused on absolute quantification of sugars, amino acids, nucleotides, organic acids, and amines.

Main Results:

  • Successfully developed a method for absolute quantification of ~100 polar metabolites.
  • Demonstrated the capability to analyze diverse metabolite classes including sugars, amino acids, and nucleotides.
  • The method is suitable for targeted metabolomics studies.

Conclusions:

  • The described HILIC-UHPLC-MS/MS method enables absolute quantification of a wide range of polar metabolites.
  • This approach is valuable for investigating biochemical responses to pathophysiological stimuli or genetic modifications.
  • The method supports comprehensive targeted metabolomics research.