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Updated: Feb 11, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
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Portable lensless wide-field microscopy imaging platform based on digital inline holography and multi-frame pixel

Antonio C Sobieranski1,2,3, Fatih Inci4, H Cumhur Tekin4

  • 1Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Division of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Light, Science & Applications
|April 17, 2018
PubMed
Summary

This study presents a lensless wide-field microscopy platform combining digital holography and super-resolution. It achieves 1.55 µm resolution for imaging microscopic biological samples like sperm and platelets.

Keywords:
digital in-line holographyimage registrationlensless imagingpixel super-resolutionpoint-of-care platform

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Area of Science:

  • Microscopy
  • Optical Imaging
  • Computational Imaging

Background:

  • Traditional microscopy faces limitations in resolution and field-of-view for biological samples.
  • Lensless imaging offers a compact alternative but often requires advanced computational techniques for high resolution.

Purpose of the Study:

  • To develop an irregular displacement-based lensless wide-field microscopy platform.
  • To enhance spatial resolution using computational pixel super-resolution with multi-frame processing.

Main Methods:

  • Combining digital in-line holography with a multi-frame pixel super-resolution approach.
  • Utilizing a hybrid method for displacement resolution: feature-based registration and continuous optimization.
  • Applying numerical phase-retrieval to reconstruct sample morphology.

Main Results:

  • Achieved a spatial resolution of 1.55 µm.
  • Demonstrated imaging capability on biological samples like sperm and platelets (micron-scale).
  • Operated on a wide field-of-view of approximately 30 mm².

Conclusions:

  • The presented lensless microscopy platform effectively combines digital holography and super-resolution for enhanced biological imaging.
  • The multi-frame processing and hybrid displacement resolution method significantly improve spatial resolution.
  • This technique offers a promising approach for high-resolution, wide-field imaging of microscopic biological structures.