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[PMNDTR mice: a new model to study neutrophils in vivo]

Caitlin M Gillis1, Laurent L Reber1

  • 1Institut Pasteur, Département d'immunologie, Inserm U1222, Unité des anticorps en thérapie et pathologie, 25, rue du Docteur Roux, 75015 Paris, France.

Medecine Sciences : M/S
|April 17, 2018
PubMed

Insights

Neutrophils protect against lethal endotoxin shock. A new mouse model allows selective neutrophil depletion, revealing their crucial protective role in systemic inflammation and offering advantages over older methods.

Area of Science:

  • Immunology
  • Host Defense Mechanisms
  • Inflammation Biology

Background:

  • Neutrophils are critical for pathogen defense but can also cause tissue injury during inflammation.
  • Existing methods for studying neutrophils in vivo, like genetic modification or antibody depletion, have limitations.
  • The role of neutrophils in adaptive immunity and inflammation resolution is an emerging area of research.

Purpose of the Study:

  • To introduce and validate a novel mouse model (PMNDTR) for selective neutrophil depletion.
  • To investigate the in vivo role of neutrophils in endotoxin-induced systemic shock using the new model.
  • To highlight the advantages of the PMNDTR model compared to traditional neutropenia models.

Main Methods:

  • Development of the PMNDTR mouse model, enabling neutrophil depletion via diphtheria toxin injection.
  • Utilizing the PMNDTR model to study the effects of neutrophil depletion on endotoxin-induced systemic shock.
  • Comparative analysis of the PMNDTR model against established neutropenia models.

Main Results:

  • Demonstrated that neutrophils play a significant protective role during lethal endotoxin-induced systemic shock.
  • The PMNDTR mouse model allows for precise and selective depletion of neutrophils.
  • The new model offers substantial advantages over classical methods for studying neutrophil function in vivo.

Conclusions:

  • Neutrophils are essential for host survival during severe inflammatory responses like endotoxin shock.
  • The PMNDTR mouse model is a valuable tool for dissecting neutrophil functions in various physiological and pathological contexts.
  • This model facilitates a deeper understanding of neutrophil contributions to both host defense and inflammatory diseases.

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