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Related Concept Videos

Locus of Control01:26

Locus of Control

229
Locus of control describes how individuals perceive the causes of events in their lives, influencing motivation and well-being. Introduced by Julian Rotter in 1954, it is categorized into internal and external locus of control.Internal Locus of ControlIndividuals with an internal locus of control believe their actions determine outcomes, fostering responsibility, self-efficacy, and motivation. For example, an employee may attribute career success to hard work. Research links this mindset to...
229
Root-Locus Method01:19

Root-Locus Method

520
A cruise control system in a car is designed to maintain a specified speed automatically by adjusting the gas pedal. The system continuously measures the vehicle's speed and makes fine adjustments to the pedal to achieve this goal. The root locus method is particularly useful for understanding how the cruise control system's behavior changes under varying conditions, such as when the car goes uphill, downhill, or faces strong wind resistance.
This system can be represented by a block...
520
Construction of Root Locus01:15

Construction of Root Locus

423
The construction of a root locus involves several key steps to analyze and visualize the behavior of a system's poles with varying gain. The number of branches in the root locus equals the number of closed-loop poles and is symmetrical about the real axis.
For positive gain values, the root locus exists on the real axis to the left of an odd number of finite open-loop poles or zeros. The root locus starts at the open-loop poles and traces the paths of the closed-loop poles as the gain...
423
Properties of the Root Locus01:05

Properties of the Root Locus

308
The root locus method is an invaluable tool for analyzing higher-order systems without needing to factor the denominator of the transfer function. A pole of the system is identified when the characteristic polynomial in the transfer function's denominator equals zero.
To determine if a point lies on the root locus, the criterion involves the sum of angles contributed by all poles and zeros to that point. Specifically, this sum must be an odd multiple of 180 degrees. The gain at any point on...
308
Rotter's Locus of Control01:14

Rotter's Locus of Control

980
Julian Rotter introduced the concept of locus of control, a cognitive factor that significantly influences personality development and learning. Locus of control refers to an individual's beliefs about the extent of control they have over events in their lives. According to Rotter, this belief system can be categorized into two types: internal and external locus of control.
Individuals with an internal locus of control believe that their personal efforts and decisions directly affect their...
980
Plotting and Calibrating the Root Locus01:19

Plotting and Calibrating the Root Locus

482
Root loci often diverge as system poles shift from the real axis to the complex plane. Key points in this transition are the breakaway and break-in points, indicating where the root locus leaves and reenters the real axis. The branches of the root locus form an angle of 180/n degrees with the real axis, where n is the number of branches at a breakaway or break-in point.
The maximum gain occurs at the breakaway points between open-loop poles on the real axis, while the minimum gain is...
482

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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Endogenous Locus Reporter Assays.

Yaping Liu1, Jeffrey Hermes2, Jing Li1

  • 1Screening & Protein Sciences, Merck Research Labs, Merck & Co., Inc., West Point, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2018
PubMed
Summary
This summary is machine-generated.

This study details building reporter gene assays for high-throughput screening (HTS). Endogenous locus reporters offer more accurate gene expression monitoring than traditional exogenous assays for compound screening.

Keywords:
Endogenous locus reporterExogenous reporterHigh-throughput screening (HTS)LuciferaseMycNanoLucPESTReporter gene

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Drug Discovery

Background:

  • Reporter gene assays are crucial for high-throughput screening (HTS) to identify compounds affecting gene expression.
  • Traditional exogenous reporter assays use cloned regulatory elements, which may not fully reflect endogenous gene regulation.
  • Endogenous locus reporters insert a reporter gene into the native gene locus for more accurate transcriptional monitoring.

Purpose of the Study:

  • To introduce considerations for developing reporter gene assays for HTS.
  • To describe methods for establishing 1536-well format endogenous and exogenous reporter assays.
  • To screen compounds modulating Myc pathway activity.

Main Methods:

  • Development of endogenous locus reporter assays.
  • Development of exogenous reporter assays.
  • Establishment of 1536-well assay formats for HTS.
  • Screening for compounds affecting Myc pathway activity.

Main Results:

  • Successfully established 1536-well format endogenous and exogenous reporter assays.
  • Demonstrated the utility of these assays for compound screening.
  • Identified compounds modulating Myc pathway activity.

Conclusions:

  • Endogenous locus reporter assays provide a more accurate reflection of gene regulation compared to exogenous assays.
  • The developed HTS assays are effective tools for identifying modulators of specific biological pathways, such as the Myc pathway.