Related Experiment Video
Updated: Feb 11, 2026
06:46
In Vivo Functional Assessment of Rat Masseter Muscle Following Surgical Creation of a Volumetric Muscle Loss (VML) Injury
Published on: November 15, 2024
957
50 Hz volumetric functional imaging with continuously adjustable depth of focus
Rongwen Lu1,2, Masashi Tanimoto1,3,2, Minoru Koyama1
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Biomedical Optics Express
|April 21, 2018
Summary
This study introduces a cost-effective Bessel beam module for advanced microscopy. It enables faster 3D neural activity monitoring in vivo by increasing imaging depth without sacrificing resolution.
Area of Science:
- Neuroscience
- Optical Microscopy
- Biophysics
Background:
- Monitoring large neural populations is crucial for understanding behavior.
- Existing methods often struggle with high spatial resolution and volume rates.
Purpose of the Study:
- To develop an adaptable and cost-effective module for enhanced 3D neural circuit monitoring.
- To increase the volume imaging rate of two-photon fluorescence microscopes.
Main Methods:
- An axicon-based Bessel beam module with continuously adjustable depth of focus (CADoF) was developed.
- The module extends excitation focus axially, maintaining lateral resolution.
- It was integrated into existing two-photon microscopes.
Main Results:
- The CADoF Bessel module effectively converts frame rate into volume rate.
- Simultaneous monitoring of spinal projection neurons in larval zebrafish was achieved.
- Activity was recorded over 60 µm depth at 50 Hz volume rate with adjustable axial extent.
Conclusions:
- The CADoF Bessel module offers a compact and cost-effective solution for high-speed 3D neural imaging.
- This technology facilitates deeper insights into neural circuit function and behavior control.
Keywords:
(110.0110) Imaging systems(170.2520) Fluorescence microscopy(170.2655) Functional monitoring and imaging(180.0180) MicroscopyMore Related Videos
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