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Updated: Feb 11, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
A Label-Free Detection of NdeI Endonuclease Activity by Using DNA-Templated Silver Nanoclusters
Chunghyun Lee1, Jongback Gang1
1Department of Nano Chemistry, Gachon University, Seongnam-si, Gyeonggi-do 13120, South Korea.
Abstract:
Restriction endonucleases play an important role in genetic recombination, molecular cloning, clinical diagnosis, and pharmacological application in drug studies. In the present study, DNA-templated Ag+ nanoclusters (DNA-AgNCs) were used to detect NdeI endonuclease activity due to their facile synthesis, outstanding optical properties, and good biocompatibility. This study monitored fluorescence intensity of DNA-AgNCs to detect NdeI endonuclease activity and its inhibition in the presence of 5-fluorouracil under optimal conditions.
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