Related Experiment Video
Updated: Jan 25, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
Published on: October 6, 2017
Label-Free Detection of DNA Polymerase Activity Using Hairpin DNA-Templated Silver Nanoclusters
1Department of Nano Chemistry, Gachon University, Sungnam, 13120, South Korea.
Abstract:
Based on the characteristic properties like strong fluorescence and high photostability, DNA-templated silver nanoclusters (DNA-AgNCs) were utilized to detect Klenow fragment exo- (KF-) activity and to monitor its inhibition by daidzein. In this study, fluorescence intensity is linearly correlated for KF- concentration ranging from 0 to 2.0 U/mL, with a minimum detectable concentration of 0.05 U/mL. In addition, KF- inhibition was monitored by changes in fluorescence intensity with increasing daidzein concentration gradually.
Related Concept Videos
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
DNA as a Genetic Template
DNA as a Genetic Template
From DNA to Protein
DNA Replication
Replication in Prokaryotes
DNA replication...
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

