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Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
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Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

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Circular RNA circNASP modulates the malignant behaviors in osteosarcoma via miR-1253/FOXF1 pathway

Lipeng Huang1, Mangmang Chen1, Jun Pan2

  • 1Department of Orthopaedic Surgery, Wenzhou Central Hospital, The DingLi Clinical Institute of Wenzhou Medical University, Wenzhou 325000, PR China.

Biochemical and Biophysical Research Communications
|April 22, 2018
PubMed

Insights

Circular RNAs (circRNAs) are key in cancer. This study reveals circNASP promotes osteosarcoma (OS) growth and spread by sponging miR-1253, increasing FOXF1 expression, suggesting a new therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized as crucial regulators in human diseases, particularly cancer.
  • Research into circRNA function in osteosarcoma (OS) is a developing field.

Purpose of the Study:

  • To investigate the role of a novel circRNA, circNASP, in osteosarcoma.
  • To elucidate the underlying molecular mechanism of circNASP in OS progression.

Main Methods:

  • Screening and quantification of circNASP expression in OS tissues.
  • Functional assays including circNASP knockdown and rescue experiments.
  • Mechanism investigation involving miRNA sponging and target gene analysis (miR-1253 and FOXF1).

Main Results:

  • circNASP was significantly upregulated in OS tissues and correlated with tumor size and metastasis.
  • circNASP knockdown inhibited OS cell proliferation, cell cycle progression, and invasion.
  • circNASP functions as a miR-1253 sponge, upregulating FOXF1 expression.

Conclusions:

  • circNASP promotes malignant behaviors in osteosarcoma cells via the circNASP/miR-1253/FOXF1 pathway.
  • The circNASP/miR-1253/FOXF1 axis represents a potential therapeutic target for osteosarcoma.
Keywords:
Cell cycleInvasionOsteosarcomaProliferationcircNASP

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