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Layer-by-Layer Engineered Polymer Capsules for Therapeutic Delivery.

Rona Chandrawati1

  • 1School of Chemical Engineering, The University of New South Wales, Sydney, NSW, 2052, Australia. rona.chandrawati@unsw.edu.au.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2018
PubMed
Summary
This summary is machine-generated.

Layer-by-layer assembly creates polymer capsules for gene delivery. These DNA-loaded capsules degrade in cells, releasing therapeutic cargo effectively.

Keywords:
DNADisulfide bondDrug deliveryLayer-by-layerPoly(ethylene glycol)Poly(methacrylic acid)Polymer capsulesRedox-responsiveSurface conjugation

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Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Layer-by-layer (LbL) assembly offers versatile fabrication of polymer capsules for drug delivery.
  • The choice of materials in LbL assembly dictates capsule properties and functionalities.

Purpose of the Study:

  • To describe protocols for fabricating LbL-engineered poly(methacrylic acid) (PMA) capsules for gene delivery.
  • To detail methods for synthesis, cargo encapsulation, multilayer formation, surface modification, and cross-linking.

Main Methods:

  • DNA adsorption onto silica particle templates.
  • Formation of hydrogen-bonded multilayers using thiol-functionalized PMA and poly(N-vinylpyrrolidone) (PVP).
  • Surface modification with PMA-poly(ethylene glycol) (PEG) copolymers, followed by film stabilization and template dissolution.

Main Results:

  • Successful fabrication of disulfide-cross-linked DNA-loaded PMA capsules.
  • Capsules designed for degradation and cargo release in intracellular reducing environments.
  • Demonstration of LbL assembly for creating functional therapeutic carriers.

Conclusions:

  • LbL-assembled PMA capsules are effective carriers for gene delivery.
  • The developed protocols enable the creation of stimuli-responsive drug delivery systems.
  • These capsules offer a promising platform for intracellular therapeutic applications.