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Published on: December 8, 2023
Proteus vulgaris - Pt electrode system for urea to nitrogen conversion in synthetic urine
Myreisa Morales-Cruz1, Marjorie López-Nieves1, Roberto Morales-Hernández1
1Department of Chemistry, NSF-CREST Center for Innovation, Research and Education in Environmental Nanotechnology (CIRE2N), Molecular Sciences Research Center, University of Puerto Rico, Río Piedras Campus, San Juan 00931, Puerto Rico.
Abstract:
One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in ~2.5×109cfu/mL Proteus vulgaris in synthetic urine.
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