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Synergistic Antibacterial Activity of a New Quinolone, T-3762, and Human Gamma Globulin Against Intracellular
Satonori Kurashige1, Mariko Nakamura1, Yuki Akuzawa1
1Department of Laboratory Sciences, Gunma University School of Health Sciences, Maebashi, Japan.
Abstract:
The bactericidal activity of antibacterial agents against intracellular bacteria using a Salmonella-lined macrophage model (TtT/M-87), established from mouse pituitary tumor-associated macrophages, was investigated in this study. Because a virulent strain of Salmonella enteritidis 116-54 is able to proliferate in phagocytized macrophages, the bactericidal activity against intracellular bacteria can be determined using this model. We found that a new quinolone, T-3762, has strong bactericidal activity against virulent S. enteritidis in macrophages. The bactericidal activity of T-3762 was augmented by the addition of human γ-globulin. The combination of T-3762 and human γ-globulin showed significantly higher bactericidal activity than that of ofloxacin and human γ-globulin. These results suggest that T-3762 can penetrate mammalian cells in an active form and that human γ-globulin may be able to enhance the bactericidal activity of macrophages by opsonization of bacteria with antibody in the γ-globulin fraction.
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