Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

4.0K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
4.0K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.6K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.6K
Direct-Acting Cholinergic Agonists: Pharmacokinetics01:31

Direct-Acting Cholinergic Agonists: Pharmacokinetics

1.9K
Direct-acting cholinergic agonists, such as synthetic choline esters and naturally occurring alkaloids, exert their effects by enhancing the actions of acetylcholine and stimulating the parasympathetic nervous system. Synthetic choline esters share structural similarities with acetylcholine. For example, they have a positively charged quaternary ammonium or onium group, contributing to their hydrophilic characteristics. As a result, they are poorly absorbed in the body through oral...
1.9K
Drugs Acting on Autonomic Ganglia: Stimulants01:23

Drugs Acting on Autonomic Ganglia: Stimulants

2.1K

Ganglionic stimulants activate NM nicotinic receptors in autonomic ganglia, falling into two categories: nicotine mimetics [e.g., lobeline, dimethylpiperazine, tetramethylammonium] and muscarinic receptor agonists [e.g., muscarine, methacholine]. The first category's action is rapid and blocked by nicotinic receptor antagonists, while the second category's action is delayed and blocked by atropine-like agents. Nicotine, an alkaloid, affects the heart rate by stimulating...
2.1K
Drugs Acting on Autonomic Ganglia: Blockers01:28

Drugs Acting on Autonomic Ganglia: Blockers

1.7K
Ganglionic blockers inhibit autonomic activity by blocking nicotinic receptors in the autonomic ganglia, suppressing impulse transmission. These blockers lack selectivity between sympathetic and parasympathetic ganglia and are ineffective as neuromuscular junction antagonists. They can be categorized into two groups:
1.7K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure basis for single-strand nucleic acid targeting by IscB and variants.

Nucleic acids research·2026
Same author

Structure basis for single-strand nucleic acid targeting by IscB and variants.

bioRxiv : the preprint server for biology·2026
Same author

Assessing and engineering the IscB-ωRNA system for programmed genome editing.

Nature chemical biology·2024
Same author

Craspase is a CRISPR RNA-guided, RNA-activated protease.

Science (New York, N.Y.)·2022
Same author

Structural basis for RNA-guided DNA cleavage by IscB-ωRNA and mechanistic comparison with Cas9.

Science (New York, N.Y.)·2022
Same author

Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.

Nature·2021
Same journal

SqueakPose Studio, an end-to-end platform for pose estimation and real-time edge-AI deployment.

eLife·2026
Same journal

Mechanistic insights into transcriptional regulation of ARHGAP36 expression identify a factor predictive of neuroblastoma survival.

eLife·2026
Same journal

Activity-dependent CO<sub>2</sub> production in the axon triggers opening of Connexin32 in the Schwann cell paranode.

eLife·2026
Same journal

Lipid packing contributes to the confinement of caveolae to the plasma membrane.

eLife·2026
Same journal

A coma pattern-based autofocusing method resolves bacterial cold shock response at single-cell level.

eLife·2026
Same journal

Non-canonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy.

eLife·2026
See all related articles

Related Experiment Video

Updated: Feb 11, 2026

Ambient Method for the Production of an Ionically Gated Carbon Nanotube Common Cathode in Tandem Organic Solar Cells
14:37

Ambient Method for the Production of an Ionically Gated Carbon Nanotube Common Cathode in Tandem Organic Solar Cells

Published on: November 5, 2014

9.9K

Acting in tandem.

Robert A Battaglia1, Ailong Ke1

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, United States.

Elife
|April 24, 2018
PubMed
Summary
This summary is machine-generated.

Tandem riboswitches enable bacteria to perform complex logic during nutrient scarcity. These RNA structures act as molecular switches, controlling gene expression in response to environmental cues.

Keywords:
B. subtilisPRPPaptamerbiochemistrychemical biologygene regulationnoncoding RNAphosphoribosyl pyrophosphate

More Related Videos

Identification of Protein Interacting Partners Using Tandem Affinity Purification
10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

38.3K
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Published on: January 26, 2017

15.6K

Related Experiment Videos

Last Updated: Feb 11, 2026

Ambient Method for the Production of an Ionically Gated Carbon Nanotube Common Cathode in Tandem Organic Solar Cells
14:37

Ambient Method for the Production of an Ionically Gated Carbon Nanotube Common Cathode in Tandem Organic Solar Cells

Published on: November 5, 2014

9.9K
Identification of Protein Interacting Partners Using Tandem Affinity Purification
10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

38.3K
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Published on: January 26, 2017

15.6K

Area of Science:

  • Bacterial molecular biology
  • RNA structure and function
  • Gene regulation

Background:

  • Bacteria utilize regulatory RNA elements to sense and respond to environmental changes.
  • Riboswitches are key regulatory elements that modulate gene expression.
  • Tandem riboswitches represent a more complex class of regulatory RNA structures.

Purpose of the Study:

  • To investigate the functional capabilities of tandem riboswitches in bacteria.
  • To elucidate the mechanisms by which tandem riboswitches perform logical operations.
  • To understand the role of these RNA structures in bacterial adaptation to nutrient starvation.

Main Methods:

  • Computational modeling of RNA structures and interactions.
  • In vitro biochemical assays to characterize riboswitch activity.
  • Genetic manipulation of bacterial strains to assess in vivo function.

Main Results:

  • Tandem riboswitches can integrate multiple environmental signals to control gene expression.
  • These RNA structures exhibit complex logical behaviors, such as AND and OR gates.
  • The activity of tandem riboswitches is crucial for bacterial survival under nutrient-limited conditions.

Conclusions:

  • Tandem riboswitches provide bacteria with sophisticated regulatory circuits for nutrient sensing.
  • These RNA structures expand our understanding of bacterial adaptive strategies.
  • Tandem riboswitches represent a promising target for synthetic biology applications.