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Related Experiment Video

Updated: Feb 11, 2026

Simplified Human Neutrophil Extracellular Traps NETs Isolation and Handling

Simplified Human Neutrophil Extracellular Traps NETs Isolation and Handling

Published on: April 16, 2015

Cytokine Network & NETs.

M Garley¹, E Jabloňská¹, A Suraźyński²

¹Department of Immunology, Medical University of Bialystok, Białystok, Poland.

Folia Biologica

|April 25, 2018

Summary

This summary is machine-generated.

Pro-inflammatory cytokines IL-15, IL-17, and IL-18, along with fMLP, rapidly induce neutrophil extracellular traps (NETs). Anti-inflammatory IL-10 delays NET formation, while all tested cytokines affect myeloperoxidase (MPO) expression in neutrophils.

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Simplified Human Neutrophil Extracellular Traps NETs Isolation and Handling

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Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

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Published on: March 2, 2014

Area of Science:

Immunology
Cell Biology

Background:

Neutrophil extracellular traps (NETs) are crucial in host defense but can also contribute to inflammatory diseases.
Cytokines are key regulators of immune responses, influencing neutrophil function and NET formation.

Purpose of the Study:

To investigate the effects of specific pro-inflammatory (IL-15, IL-17, IL-18) and anti-inflammatory (IL-10) cytokines on NET formation.
To compare the impact of these cytokines with known NET inducers like IL-8, lipopolysaccharide (LPS), and formyl-methionyl-leucyl-phenylalanine (fMLP).
To evaluate the expression of myeloperoxidase (MPO), a critical component of NETs, under various stimulation conditions.

Main Methods:

Neutrophil isolation using Polymorphprep™ and CD16 MACS® microbead sorting.
Incubation of neutrophils with selected cytokines and pathogenic factors.
Assessment of NET formation via microscopy (BD Pathway 855) and quantification of circulating DNA.
Evaluation of MPO expression using flow cytometry.

Main Results:

Recombinant human IL-15 (rhIL-15), rhIL-17, rhIL-18, and fMLP induced rapid NET formation.
Interleukin-10 (IL-10) significantly delayed NET formation.
Cytokine stimulation resulted in two distinct neutrophil populations based on MPO expression (MPOlow and MPOhigh).
LPS and fMLP stimulation identified neutrophil populations differing in MPO expression and cell size.

Conclusions:

Pro-inflammatory cytokines IL-15, IL-17, and IL-18, similar to fMLP, are potent inducers of NET formation.
IL-10 exhibits an inhibitory or delaying effect on NETosis.
Differential MPO expression and cell size heterogeneity in neutrophils are modulated by various inflammatory stimuli, suggesting complex regulatory mechanisms in NETosis.