Related Experiment Video

Updated: Feb 11, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
06:38

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

41.3K

POLYMORPHISM PREVALENCE OF TLR 9 TYPE GENE IN PATIENTS WITH CHRONIC FORMS OF EPSTEIN-BARR VIRUS-INFECTION

T Lyadova1, O Ognivenko1, O Rzhevska1

  • 1V.N. Karazin Kharkov National University; Kharkov Medical Academy of Post-Graduate Education, Ukraine.

Georgian Medical News
|April 27, 2018
PubMed

Insights

This study investigated the TLR-9 gene polymorphism in patients with chronic Epstein-Barr virus (EBV) infection. The TC genotype was found to be dominant and specifically associated with EBV infection, unlike TT and CC genotypes.

Area of Science:

  • Immunogenetics
  • Virology
  • Molecular Biology

Background:

  • Epstein-Barr virus (EBV) causes chronic infections, including chronic forms of viral Epstein-Barr (VEB).
  • Toll-like receptor 9 (TLR9) plays a crucial role in innate immunity against viral pathogens.
  • Genetic variations in TLR9 may influence susceptibility and disease progression in EBV infections.

Purpose of the Study:

  • To determine the frequency of the TLR-9 1486 T/C gene polymorphism in patients with chronic VEB infection.
  • To investigate the association between TLR-9 genotypes and Epstein-Barr virus infection.

Main Methods:

  • DNA was isolated from 44 patients with chronic VEB infection using a commercial kit and sorption assay.
  • The TLR9 gene's 1486 T/C polymorphism (rs187084) was analyzed using real-time PCR with restriction fragment-length polymorphism (RFLP).
  • Restriction enzymes (Ncol) and specific oligonucleotide primers were employed for amplification and detection.

Main Results:

  • Three genotypes (TT, TC, CC) were identified for the TLR-9 1486 T/C polymorphism.
  • The TC genotype was found to be dominant among the studied patient cohort.
  • A specific association was observed between the TC genotype and the presence of HEBV (Epstein-Barr virus), while TT and CC genotypes showed no such specificity.

Conclusions:

  • The TLR-9 1486 T/C gene polymorphism exhibits differential distribution in patients with chronic Epstein-Barr virus infection.
  • The TC genotype of TLR-9 may be a genetic marker associated with susceptibility or a specific immune response in HEBV infections.
  • Further research is warranted to elucidate the functional implications of the TLR-9 TC genotype in the context of EBV pathogenesis.

More Related Videos

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line09:14

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

4.7K
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection09:43

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Published on: March 30, 2018

9.7K

Related Concept Videos

Prevalence and Incidence01:08

Prevalence and Incidence

In statistical epidemiology and health sciences, two essential metrics—prevalence and incidence—are fundamental for understanding disease dynamics within a population. These measures enable public health officials, epidemiologists, and researchers to assess the burden of diseases, allocate resources effectively, and design impactful public health policies and interventions.
Prevalence indicates the proportion of individuals in a population who have a specific disease or health...
1.9K
Pharmaceutical Alternatives: Polymorphic Form-Related and Particle Size-Related Therapeutic Nonequivalence01:27

Pharmaceutical Alternatives: Polymorphic Form-Related and Particle Size-Related Therapeutic Nonequivalence

Changes in polymorphic forms can significantly influence the bioavailability of poorly soluble drugs. Although the FDA defines pharmaceutical equivalence based on having the same active ingredient, dosage form, and route of administration, it does not automatically disqualify products with different polymorphic forms. This means two products with different polymorphs can still be deemed pharmaceutically equivalent. However, polymorphic differences can affect properties like wettability,...
179
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
31.1K
What are Viruses?00:50

What are Viruses?

Overview
128.4K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
18.5K
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.7K