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The human transcript induced in spermatogenesis 50.
Yoshihito Sasaki1, Toshinobu Miyamoto1, Kazuo Sengoku1
1Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa, Hokkaido, Japan.
Reproductive Medicine and Biology
|April 28, 2018
Summary
Researchers identified three human genes, TISP50, TISP15, and TISP43, specifically expressed in the testis. These genes are crucial for spermatogenesis, showing a difference compared to mouse Tisp genes.
Area of Science:
- Reproductive biology
- Genetics
- Molecular biology
Background:
- Specific gene expression in the testis is vital for spermatogenesis.
- Previous studies identified numerous mouse "transcripts induced in spermatogenesis" (Tisp) genes.
- Fewer human testis-specific genes have been identified compared to rodents.
Purpose of the Study:
- To isolate and characterize human genes specifically expressed in the testis.
- To investigate potential human homologs of mouse Tisp genes.
- To understand the role of these genes in human spermatogenesis.
Main Methods:
- Searched human genome for homology to mouse Tisp genes using GenBank.
- Designed primers in homologous human regions for PCR analysis.
- Screened various human tissue cDNA libraries to identify testis-specific transcripts.
Main Results:
- Analyzed 28 human TISP-related genes.
- Identified three genes (TISP50, TISP15, TISP43) specifically expressed in human testis.
- Isolated the cDNAs for these three testis-specific genes.
Conclusions:
- Significant differences exist in TISP gene families between humans and mice.
- Human TISP50, TISP15, and TISP43 genes are likely critical for spermatogenesis.
- These findings contribute to understanding male reproductive genetics.
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