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Updated: Feb 11, 2026

Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
Evaluation of specific modified histones in lip carcinogenesis
Silas Antonio Juvencio de Freitas Filho1, João Paulo Silva Servato2, Rodrigo Tavares de Sá1
1Area of Pathology, School of Dentistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
Objective:
Histones regulate chromatin density and therefore influence gene expression and cellular proliferation. These properties are modified by methylation, acetylation and phosphorylation of histones. The aim of this study was to investigate the variation of specific modified histones in actinic cheilitis (AC) and squamous cell carcinoma of the lip (SCCL).
Methods:
Samples of non-neoplastic tissue of the lip (NNTL, n = 9), AC (n = 33), and SCCL (n = 27) were submitted to immunohistochemistry to detect the modified histones H3K36me3, H3K9ac, H4K12ac, and H3S10 ph.
Results:
Reactivity for all of the modified histones was significantly decreased from NNTL to AC, but not from AC to SCCL. Dysplasia in AC or histological grade in SCCL were not related to the reactivity of any modified histones.
Conclusions:
Histone modifications are related to initial actinic damage, but not to malignant transformation in the lip.
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