[Protein metabolism during the growth of hybridoma cells]

Protein metabolism during the growth of mouse hybridoma, producing monoclonal antibodies to phage lambda has been studied. The rate of protein accumulation Va at any time of growth was equal to that of protein synthesis Vs minus the degradative rate Vd and protein secretion rate Vsecr (Vn = Vs--Vd--Vsecr). Cellular protein metabolism was described in terms of the relationship 6.3% hr = 8.1%/hr--1%/hr for the middle of exponential growth phase, and in terms of the relationship 1.4%/hr = 2.9%/hr = 0.8%/hr--0.7%/hr for the early stationary phase. The results obtained enabled us to conclude that the synthesis of cellular proteins may be the main process regulating the protein metabolism during hybridoma cell growth.