Electronic Transduction of Polymerase or Reverse Transcriptase Induced Replication Processes on Surfaces: Highly
Fernando Patolsky1, Amir Lichtenstein1, Moshe Kotler2
1Institute of Chemistry The Hebrew University of Jerusalem Jerusalem 91904 (Israel) Fax: (+972) 2-6527715.
Abstract:
Replication, precipitation, and amplification: Polymerase or reverse transcriptase induced replication of DNA/RNA on a transducer (electrode or piezoelectric crystal) leads to the ultrasensitive specific electronic transduction of viral genomes. Biotin tags (B) on the double-stranded assembly provide docking sites for a conjugate between avidin (A) and an alkaline phosphatase (AP). Enzyme biocatalysis of substrate (S) to the insoluble product (P), which precipitates onto the transducer (yellow surface), provides amplification in the analysis of the target DNA.
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