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Biomarker analysis from complex biofluids by an on-chip chemically modified light-controlled vertical nanopillar

Lanka Tata Rao1,2, Adva Raz3, Fernando Patolsky4,5

  • 1Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv-Yafo, Israel.

Nature Protocols
|January 30, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed silicon nanopillar (SiNP) arrays for rapid, reusable, and quantitative capture and release of biomarkers. This technology enables on-chip multiplex analysis of various biological and chemical species for diagnostics and drug discovery.

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Area of Science:

  • * Nanotechnology
  • * Surface Chemistry
  • * Biomolecular Engineering

Background:

  • * Nanostructured devices offer versatile applications in disease diagnostics and drug discovery.
  • * Efficient capture and release of biomarkers are crucial for sensitive and quantitative analysis.
  • * Existing methods often lack the speed, reusability, and multiplexing capabilities required for advanced applications.

Purpose of the Study:

  • * To develop a standardized protocol for producing silicon nanopillar (SiNP) arrays for biomarker capture and release.
  • * To enable rapid, reusable, and quantitative on-chip multiplex analysis of biomolecules.
  • * To demonstrate the utility of SiNP arrays for analyzing diverse biological and chemical species.

Main Methods:

  • * Fabrication of single-zone and multizone vertical silicon nanopillar arrays.
  • * Morphological characterization of SiNP arrays.
  • * Chemical modification for bioreceptor immobilization and light-induced release of captured agents.
  • * Demonstration using green fluorescent protein as a model biomarker with a microplate reader.

Main Results:

  • * A standardized protocol for SiNP array fabrication and functionalization was established.
  • * The SiNP platform demonstrated rapid, reusable, and quantitative capture and release of biomarkers.
  • * Successful multiplex capture and release of biomolecules from biosamples were achieved.
  • * The protocol can be completed within 45 hours.

Conclusions:

  • * Vertical silicon nanopillar arrays provide a versatile platform for on-chip multiplex biomarker analysis.
  • * The developed protocol facilitates the production of reusable and quantitative capture-and-release devices.
  • * This technology holds significant potential for applications in diagnostics, drug screening, and analysis of various biological and chemical species.