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Related Concept Videos

Antibody Structure01:10

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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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The specific heat capacity of a substance refers to the energy required to increase the temperature of one gram of that substance by one degree Celcius. Specific heat capacity is often represented in calories (cal), grams (g), and degrees Celsius (oC), but can also be expressed in joules (J), kilograms (kg), and Kelvin (K), among other units.
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Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
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Updated: Feb 11, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

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Antibody Specificity Profiling Using Protein Microarrays.

Pedro Ramos-López1, José Irizarry1, Ignacio Pino1

  • 1CDI Laboratories, Guanajibo Research and Innovation Park, Mayaguez, PR, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 2, 2018
PubMed
Summary
This summary is machine-generated.

Antibody specificity is crucial for accurate protein detection. This study details a protein microarray method to quantitatively profile antibody cross-reactivity, ensuring reliable biological data.

Keywords:
Antibody specificityCross-reactivityMonoclonal antibodiesProtein microarray

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Area of Science:

  • Biochemistry
  • Immunology
  • Proteomics

Background:

  • Antibodies are essential reagents for protein detection and isolation in biological research.
  • Lack of antibody specificity leads to inaccurate experimental data and misleading conclusions.
  • Quantitative profiling of antibody cross-reactivity is needed to ensure data reliability.

Purpose of the Study:

  • To describe a detailed protocol for assessing antibody specificity using protein microarrays.
  • To enable simultaneous and quantitative profiling of antibody cross-reactivity against numerous cellular antigens.

Main Methods:

  • Detailed protocol for protein microarray: array pretreatment, antibody incubation, washing, and scanning.
  • Quantitative analysis of antibody binding and cross-reactivity.
  • Simultaneous profiling of antibody interactions with a wide range of antigens.

Main Results:

  • The described protocol allows for comprehensive assessment of antibody specificity.
  • Quantitative data on antibody cross-reactivity can be obtained.
  • The method facilitates the identification of highly specific antibodies.

Conclusions:

  • Protein microarrays provide a robust platform for antibody specificity profiling.
  • This protocol enhances the reliability of protein detection and isolation studies.
  • Ensuring antibody specificity is critical for advancing biological research and diagnostics.