Related Experiment Video
Updated: Feb 11, 2026
12:28
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
Published on: November 10, 2017
9.9K
High spatial resolution detection of low-energy electrons using an event-counting method, application to point
Evelyne Salançon1, Alain Degiovanni1, Laurent Lapena1
1Aix Marseille University, CNRS, CINAM UMR 7325, F-13288 Marseille, France.
The Review of Scientific Instruments
|May 3, 2018
Summary
A new event-counting method enhances electron microscopy resolution by 7 times. This technique improves detector spatial resolution, paving the way for atomic-level imaging in electron point projection microscopy.
Area of Science:
- Physics
- Materials Science
- Microscopy
Background:
- Low-energy electron point projection microscopy (LEEP) is a powerful imaging technique.
- Improving detector spatial resolution is crucial for advancing microscopy capabilities.
Purpose of the Study:
- To implement an event-counting method using a two-microchannel plate (MCP) stack in LEEP.
- To enhance the spatial resolution and overall performance of LEEP.
Main Methods:
- Utilized a two-microchannel plate stack for event counting in LEEP.
- Demonstrated a detector spatial resolution of 15 μm, defined by the distance between first-neighbor microchannels.
Main Results:
- Achieved a 7-fold improvement in microscope resolution.
- Observed that the detector's local response is primarily influenced by the angle between the MCP hexagonal structures.
- Successfully increased the source-object distance to 350 nm from 50 nm.
Conclusions:
- The implemented event-counting method significantly enhances LEEP resolution.
- The findings suggest potential for achieving atomic resolution in electron microscopy.
- This technique offers a pathway to improved imaging of nanoscale structures.
More Related Videos
Related Concept Videos
01:12Conservation of Energy: Application
8.3K
When solving problems using the energy conservation law, the object (system) to be studied should first be identified. Often, in applications of energy conservation, we study more than one body at the same time. Second, identify all forces acting on the object and determine whether each force doing work is conservative. If a non-conservative force (e.g., friction) is doing work, then mechanical energy is not conserved. The system must then be analyzed with non-conservative work. Third, for...
8.3K
01:04Application of the Energy Equation
1.2K
The application of the energy equation to centrifugal pumps is a fundamental principle in fluid dynamics and engineering. In this scenario, the energy equation is used to calculate the flow rate of a centrifugal pump responsible for transferring water between two reservoirs at different elevations. The pump applies an energy input of 7500 joules per second, and the vertical difference between the lower and upper reservoirs is 10 meters. Additionally, the head loss due to friction and other...
1.2K
01:25Overview of Electron Microscopy
15.4K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
15.4K
01:07Scanning Electron Microscopy
5.6K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Fundamental Principles
Accelerated...
5.6K
01:15Transmission Electron Microscopy
7.3K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.3K
01:20Immunogold Electron Microscopy
5.5K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.5K