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Quercetin in equine frozen semen
J S Filho1, C D Corcini2, F C C Santos1
1Pos-Graduacao em Veterinaria, Faculdade de Veterinaria, Universidade Federal de Pelotas, Campus Universitário, Pelotas, Brasil.
Cryo Letters
|May 8, 2018
Summary
Quercetin supplementation did not improve outcomes in equine semen cryopreservation. Adding quercetin to freezing extenders did not significantly alter sperm motility, membrane integrity, or DNA integrity after thawing.
Area of Science:
- Veterinary Science
- Reproductive Biology
- Cryobiology
Background:
- Equine semen cryopreservation commonly uses sperm diluents to mitigate freeze-thaw damage.
- Optimizing cryoprotective extenders is crucial for maintaining sperm viability.
Purpose of the Study:
- To determine the optimal quercetin concentration for equine freezing extenders.
- To evaluate quercetin's protective effects on stallion spermatozoa during cryopreservation.
Main Methods:
- Quercetin was added to equine sperm freezing diluent at concentrations of 0.25, 0.5, 0.75, and 1 mM.
- Spermatozoa were analyzed post-thaw using flow cytometry.
- Statistical analysis compared control and quercetin-treated groups.
Main Results:
- No significant differences were observed in seminal parameters between control and quercetin-supplemented groups.
- Flow cytometry data showed no improvement in sperm quality metrics with quercetin.
Conclusions:
- Quercetin supplementation within the tested range (0.25–1 mM) did not enhance post-thaw equine semen quality.
- The tested concentrations of quercetin did not impact progressive motility, mitochondrial function, acrosome reaction, membrane integrity, or DNA fragmentation.
