Related Experiment Video
Updated: Feb 10, 2026

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
Published on: October 20, 2016
A multiplex HRMS assay for quantifying selected human plasma bile acids as candidate OATP biomarkers
Brian Rago1, Brendan Tierney1, A David Rodrigues1
1Pfizer Global Research & Development, Medicinal Sciences, Groton, CT, 06340, USA.
Aim:
Selected bile acids (BAs) in plasma have been proposed as endogenous probes for assessing drug-drug interactions involving hepatic drug transporters such as the organic anion-transporting polypeptides (OATP1B1 and OATP1B3).
Materials & Methods:
Plasma extracts were analyzed for selected BAs using a triple TOF API6600 high-resolution mass spectrometer.
Results:
Glycodeoxycholic acid 3-sulfate, glycochenodeoxycholic acid 3-sulfate, glycodeoxycholic acid 3-O-β-glucuronide and glycochenodeoxycholic acid 3-O-β-glucuronide are presented as potential OATP1B1/3 biomarkers.
Conclusion:
Six BAs are quantified in human plasma using a multiplexed high-resolution mass spectrometry method. Glycodeoxycholic acid 3-sulfate and glycodeoxycholic acid 3-O-β-glucuronide are proposed as potential biomarkers based on observed four- to fivefold increase in plasma AUC (vs placebo), following administration of a compound known to present as an OATP1B1/3 inhibitor in vitro.
More Related Videos
Related Concept Videos
Bile
Bile is released when dietary fats enter...
High-Resolution Mass Spectrometry (HRMS)
Quantifying Work
What is Natural Selection?
Antibiotic Selection
Types of Selection

