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Exosomes Secreted by Normoxic and Hypoxic Cardiosphere-derived Cells Have Anti-apoptotic Effect

Helia Namazi1,2, Iman Namazi3, Parisa Ghiasi4

  • 1Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Iranian Journal of Pharmaceutical Research : IJPR
|May 15, 2018
PubMed

Insights

Normoxic exosomes derived from cardiosphere-derived cells (CDCs) significantly reduced apoptosis in cardiomyocytes. These exosomes show potential for treating ischemic heart disease by protecting heart cells.

Area of Science:

  • Cardiovascular Research
  • Stem Cell Biology
  • Extracellular Vesicles

Background:

  • Cardiosphere-derived cells (CDCs) are a promising stem cell source for cardiac repair.
  • Exosomes mediate the regenerative and cardioprotective effects of CDCs, including anti-apoptotic actions.
  • Understanding exosome preparation conditions is crucial for optimizing therapeutic potential.

Purpose of the Study:

  • To investigate the anti-apoptotic effects of exosomes derived from normoxic and hypoxic CDCs.
  • To evaluate the impact of these exosomes on induced apoptosis in human embryonic stem cell-derived cardiomyocytes (hESC-CMs).

Main Methods:

  • CDCs were cultured under normoxic (18% O2) and hypoxic (1% O2) conditions.
  • CDC-derived exosomes were isolated using differential ultracentrifugation.
  • Apoptosis was induced in hESC-CMs using cobalt chloride, and caspase-3/7 activity was measured.

Main Results:

  • Normoxic exosomes (N-exo) significantly reduced caspase-positive hESC-CMs from 30.63% to 1.65% (25 μg/mL) and 1.1% (50 μg/mL).
  • Hypoxic exosomes (H-exo) reduced caspase-positive cells by 8.75% (25 μg/mL) and 12.86% (50 μg/mL).
  • N-exo demonstrated significantly higher anti-apoptotic activity compared to H-exo.

Conclusions:

  • CDC-derived exosomes inhibit cardiomyocyte apoptosis, promoting cell survival.
  • Normoxic exosome preparation enhances anti-apoptotic efficacy.
  • CDC-exosomes represent a potential therapeutic strategy for ischemic cardiac diseases.

Keywords:
Anti-apoptotic effectCardiosphere-derived cellsExosomesHypoxia preconditioning

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