Related Experiment Video
Updated: Feb 10, 2026
17:16
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
14.4K
Electrochemical Methodologies for the Detection of Pathogens
Mandana Amiri1, Abolfazl Bezaatpour1, Hamed Jafari1
1Department of Chemistry , University of Mohaghegh Ardabili , Ardabil , Iran.
ACS Sensors
|May 15, 2018
Summary
Developing rapid, sensitive, and cost-effective electrochemical sensors is crucial for early detection of pathogenic bacteria, addressing a significant global health challenge and improving food and water safety worldwide.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Microbiology
Background:
- Bacterial infections cause significant global morbidity and mortality, with limited progress in reducing deaths.
- Current detection methods (culturing, PCR, immunoassays) are time-consuming, labor-intensive, and costly.
- Pathogen detection is critical for public health, food safety, and socioeconomic stability.
Purpose of the Study:
- To review current electrochemical-based microorganism recognition approaches for pathogen detection.
- To contextualize electrochemical sensors against traditional methods like culturing and Polymerase Chain Reaction (PCR).
- To highlight recent advancements and future perspectives in rapid, sensitive, and specific pathogen detection.
Main Methods:
- Review of electrochemical sensors for detecting foodborne and waterborne pathogens.
- Comparison with conventional culturing and molecular methods (PCR).
- Highlighting microfluidic devices and immunomagnetic separation for multiplexed pathogen analysis.
Main Results:
- Electrochemical sensors offer label-free, highly sensitive detection of pathogens.
- Microfluidic and immunomagnetic separation techniques enable simultaneous analysis of multiple pathogens.
- Significant challenges remain in achieving selective, sensitive, rapid, cost-effective, and broadly applicable pathogen detection.
Conclusions:
- Electrochemical sensors show great promise for rapid and sensitive pathogen detection.
- Integration with microfluidics and immunomagnetic separation enhances capabilities for multi-analyte detection.
- Further research is needed to overcome limitations and develop universally applicable, affordable pathogen detection solutions.
Related Concept Videos
01:26What is an Electrochemical Gradient?
128.4K
Adenosine triphosphate, or ATP, is considered the primary energy source in cells. However, energy can also be stored in the electrochemical gradient of an ion across the plasma membrane, which is determined by two factors: its chemical and electrical gradients.
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
128.4K
01:06Interfacial Electrochemical Methods: Overview
907
Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current...
907
01:16Response Surface Methodology
674
Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
The process of RSM involves several key steps:
The process of RSM involves several key steps:
674
02:26Defenses Against Pathogens and Herbivores
29.7K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
29.7K
01:31Defense Against Bacterial Pathogens
2.9K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.9K
01:21Electrochemical Gradient and Channel Proteins: An Overview
4.8K
An electrochemical gradient is a fundamental concept in biology and chemistry. It regulates the movement of ions across cell membranes. This movement is influenced by two factors:
The electrical gradient: The electrical gradient across cell membranes refers to the difference in electric charge between the inside and outside of a cell. This difference drives the movement of ions towards or away from the cells. For instance, if the inside of the cell is more negatively charged relative to...
The electrical gradient: The electrical gradient across cell membranes refers to the difference in electric charge between the inside and outside of a cell. This difference drives the movement of ions towards or away from the cells. For instance, if the inside of the cell is more negatively charged relative to...
4.8K