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[Study of the Determination Method of Protein Content in Chitosan]
Objective:
Using the Coomassie briliant blue method to solve the problem of the precipitation when detecting the protein content in chitosan.
Methods:
Improving the Coomassie briliant blue method.
Results:
Use the Coomassie briliant blue solution with hydrochloric acid concentration of 5% as stain, stain 15 min , the maximum absorption wavelength is 598 nm. the standard curve of absorbance-protein concentration isy=0.003x+0.017 9.r2=0.991, the linear correlation is good within the protein concentration scope of 5μg/mL~40μg/mL, the lowest detection limit is 1μg/mL, and its recovery is 97.8%~103.7%.
Conclusions:
In this paper, the method of determining the content of protein in chitosan is high accurate, stable, reliable and reproducible, and can be widely used in product testing.
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