Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Response Surface Methodology01:16

Response Surface Methodology

638
Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
The process of RSM involves several key steps:
638
Elastin is Responsible for Tissue Elasticity01:12

Elastin is Responsible for Tissue Elasticity

3.1K
Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
3.1K
Classifying Matter by Composition03:35

Classifying Matter by Composition

90.0K
Matter: Pure Substances and Mixtures
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures. 
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated. 
A mixture is composed of two or...
90.0K
Fibril-associated Collagen01:11

Fibril-associated Collagen

3.3K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
3.3K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.9K
Overview
79.9K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

14.7K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leadership support for an intentional interpersonal interactions (I<sup>3</sup>)-enabled promotion and tenure process.

Journal of prosthodontic research·2026
Same author

Design and Implementation of a Structured Summer Research Program to Integrate Mentoring and Professional Development in Dental Education.

Journal of dental education·2026
Same author

Using Citric-Acid-Based Anodization to Form Magnesium-Doped Carbonated Apatite-Containing Oxides on Solid and 3D-Printed Titanium Substrates.

Journal of functional biomaterials·2026
Same author

A strategic framework for promotion and tenure in academic dentistry.

Journal of prosthodontic research·2025
Same author

Organic Acid-Based Anodization Process to Produce Bioactive Oxides on Titanium Implants.

Materials (Basel, Switzerland)·2025
Same author

Recent Advances in the Applications of Biomaterials in Ovarian Cancer.

Biomimetics (Basel, Switzerland)·2025

Related Experiment Video

Updated: Jan 26, 2026

Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

944

Optimization of collagen-elastin-like polypeptide composite tissue engineering scaffolds using response surface

Bhuvaneswari Gurumurthy1, Jason A Griggs1, Amol V Janorkar1

  • 1Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, Jackson, MS 39216, United States.

Journal of the Mechanical Behavior of Biomedical Materials
|May 18, 2018
PubMed
Summary

This study reinforced collagen scaffolds with elastin-like polypeptide (ELP) to enhance mechanical properties for tissue engineering. Optimized composites show improved rigidity and reduced water content, making them suitable for regenerative applications.

Keywords:
Central composite designGuided bone regenerationMechanical propertiesModulusPhysical characteristicsRSM

More Related Videos

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
07:35

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Published on: June 9, 2014

22.4K
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
08:35

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

Published on: April 8, 2011

16.1K

Related Experiment Videos

Last Updated: Jan 26, 2026

Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

944
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
07:35

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Published on: June 9, 2014

22.4K
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
08:35

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

Published on: April 8, 2011

16.1K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Collagen scaffolds are vital for tissue regeneration but suffer from poor mechanical integrity.
  • Improving scaffold mechanical and physical properties is crucial for successful tissue engineering.

Purpose of the Study:

  • To enhance the mechanical and physical properties of collagen scaffolds by incorporating elastin-like polypeptide (ELP).
  • To optimize the collagen-ELP composite composition using response surface methodology (RSM).

Main Methods:

  • Response surface methodology (RSM) with a central composite design was employed to optimize collagen and ELP concentrations.
  • Uniaxial tensile testing was used to evaluate mechanical properties (tensile strength, elastic modulus, toughness).
  • Physical properties were characterized using swelling ratio, differential scanning calorimetry, Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM).

Main Results:

  • An optimal composite composition of 6 mg/mL collagen and 18 mg/mL ELP was identified, exhibiting superior tensile properties.
  • ELP addition reduced water content and indicated collagen-ELP interactions.
  • SEM revealed a denser microstructure in collagen-ELP composites compared to collagen-only hydrogels.

Conclusions:

  • The addition of ELP significantly improves the mechanical properties of collagen scaffolds.
  • Optimized collagen-ELP composites offer rigid, porous structures suitable for tissue engineering applications.
  • These enhanced scaffolds hold promise for advancing regenerative medicine.